Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 229135..229862 Replicon plasmid pKPN-332
Accession NZ_CP014763
Organism Klebsiella pneumoniae strain KPNIH39

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag WM47_RS27910 Protein ID WP_011251285.1
Coordinates 229135..229446 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag WM47_RS27915 Protein ID WP_011251286.1
Coordinates 229443..229862 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM47_RS27880 224884..225531 + 648 WP_040217065.1 cysteine hydrolase -
WM47_RS27885 225573..226541 + 969 WP_077268206.1 IS5 family transposase -
WM47_RS27890 226599..227648 - 1050 Protein_226 IS66 family transposase -
WM47_RS27895 227697..228044 - 348 WP_040217144.1 IS66 family insertion sequence element accessory protein TnpB -
WM47_RS27900 228041..228379 - 339 WP_023288266.1 IS66 family insertion sequence hypothetical protein -
WM47_RS27905 228472..228930 + 459 Protein_229 DDE-type integrase/transposase/recombinase -
WM47_RS27910 229135..229446 + 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
WM47_RS27915 229443..229862 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
WM47_RS27920 230009..230977 + 969 WP_077268207.1 IS5 family transposase -
WM47_RS27925 231295..232320 - 1026 WP_001101446.1 IS110 family transposase -
WM47_RS27930 232470..233021 + 552 WP_029497558.1 DUF3833 domain-containing protein -
WM47_RS27935 233018..233977 + 960 WP_040210203.1 DUF523 and DUF1722 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid formA mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / pla 1..284894 284894
- inside IScluster/Tn - - 225573..232320 6747


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T61790 WP_011251285.1 NZ_CP014763:229135-229446 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T61790 NZ_CP014763:229135-229446 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT61790 WP_011251286.1 NZ_CP014763:229443-229862 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT61790 NZ_CP014763:229443-229862 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1


Antitoxin

Source ID Structure

References