Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4951838..4952484 Replicon chromosome
Accession NZ_CP014762
Organism Klebsiella pneumoniae strain KPNIH39

Toxin (Protein)


Gene name higB Uniprot ID A0A486LFK7
Locus tag WM47_RS24865 Protein ID WP_023301313.1
Coordinates 4952137..4952484 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag WM47_RS24860 Protein ID WP_002920557.1
Coordinates 4951838..4952137 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM47_RS24840 4948044..4948802 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
WM47_RS24845 4948852..4949682 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
WM47_RS24850 4949733..4950062 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
WM47_RS24855 4950267..4951775 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
WM47_RS24860 4951838..4952137 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
WM47_RS24865 4952137..4952484 - 348 WP_023301313.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
WM47_RS24870 4952660..4955107 - 2448 WP_002920561.1 glycogen phosphorylase -
WM47_RS24875 4955125..4956558 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13506.49 Da        Isoelectric Point: 6.2327

>T61786 WP_023301313.1 NZ_CP014762:c4952484-4952137 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKETITMADREFSQHLTKER

Download         Length: 348 bp

>T61786 NZ_CP014762:c4952484-4952137 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGACGATTACTATGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT61786 WP_002920557.1 NZ_CP014762:c4952137-4951838 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT61786 NZ_CP014762:c4952137-4951838 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486LFK7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References