Detailed information of TA system TA061774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1441946..1442543
• Replicon: chromosome
• Accession: NZ_CP014762
• Organism: Klebsiella pneumoniae strain KPNIH39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: WM47_RS07155
• Protein ID: WP_004893639.1
• Coordinates: 1441946..1442263 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: WM47_RS07160
• Protein ID: WP_004142561.1
• Coordinates: 1442256..1442543 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM47_RS07140	1437646..1439073	-	1428	WP_004176980.1	MFS transporter	-
WM47_RS07145	1439182..1440048	+	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
WM47_RS07150	1440718..1441761	+	1044	WP_023279204.1	DUF2157 domain-containing protein	-
WM47_RS07155	1441946..1442263	+	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WM47_RS07160	1442256..1442543	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
WM47_RS07165	1442681..1443334	-	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
WM47_RS07170	1443454..1443822	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
WM47_RS07175	1443822..1444403	-	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
WM47_RS07180	1444583..1445698	+	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
WM47_RS07185	1445729..1446070	+	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
WM47_RS07190	1446088..1446336	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T61774 WP_004893639.1 NZ_CP014762:1441946-1442263 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T61774 NZ_CP014762:1441946-1442263 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGTCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT61774 WP_004142561.1 NZ_CP014762:1442256-1442543 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT61774 NZ_CP014762:1442256-1442543 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACGTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1