Detailed information of TA system TA061739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2420279..2420850
• Replicon: chromosome
• Accession: NZ_CP014754
• Organism: Marinobacter sp. LQ44

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A142FRV1
• Locus tag: ASQ50_RS11170
• Protein ID: WP_058090862.1
• Coordinates: 2420279..2420557 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A142FRV2
• Locus tag: ASQ50_RS11175
• Protein ID: WP_058090861.1
• Coordinates: 2420557..2420850 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASQ50_RS11135	2415862..2416611	+	750	WP_058090868.1	NAD(P)H-dependent oxidoreductase	-
ASQ50_RS11140	2416772..2417113	+	342	WP_058090867.1	ribonuclease E inhibitor RraB	-
ASQ50_RS11145	2417243..2417557	+	315	WP_058090866.1	hypothetical protein	-
ASQ50_RS11150	2417550..2417849	+	300	WP_058090865.1	helix-turn-helix domain-containing protein	-
ASQ50_RS11155	2418314..2419357	-	1044	WP_058090864.1	IS110 family transposase	-
ASQ50_RS11160	2419512..2419913	-	402	WP_054641852.1	PIN domain nuclease	-
ASQ50_RS11165	2419910..2420104	-	195	WP_058090863.1	type II toxin-antitoxin system VapB family antitoxin	-
ASQ50_RS11170	2420279..2420557	+	279	WP_058090862.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ASQ50_RS11175	2420557..2420850	+	294	WP_058090861.1	HigA family addiction module antidote protein	Antitoxin
ASQ50_RS11180	2420979..2421236	+	258	WP_022990189.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASQ50_RS11185	2421224..2421526	+	303	WP_058090860.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASQ50_RS20815	2422310..2423554	+	1245	Protein_2218	EAL domain-containing protein	-
ASQ50_RS11195	2423532..2424602	-	1071	WP_058090858.1	magnesium/cobalt transporter CorA	-
ASQ50_RS11200	2424788..2425294	+	507	WP_058090857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2409664..2421746	12082
	-	flank	IS/Tn	-	-	2418314..2419357	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10734.21 Da
• Isoelectric Point: 10.0256

>T61739 WP_058090862.1 NZ_CP014754:2420279-2420557 [Marinobacter sp. LQ44]
MIKSFRHKGLKRFYSTGNTSGIQPDHAKKLRMQLAALDTATSVEDMEIPGFRLHPLKGKDKGRWSIRVNGNWRMTFEFQD
GNAYILDYEDYH

Nucleotide

• Length: 279 bp

>T61739 NZ_CP014754:2420279-2420557 [Marinobacter sp. LQ44]
ATGATTAAATCATTCCGTCACAAAGGACTGAAACGGTTCTACTCGACTGGCAACACGTCAGGCATACAGCCTGACCACGC
CAAGAAGCTGCGCATGCAGCTGGCTGCCCTGGATACGGCGACTTCTGTGGAAGACATGGAGATCCCCGGTTTTCGGCTCC
ACCCATTGAAGGGTAAAGACAAAGGGCGGTGGTCGATACGGGTTAACGGAAACTGGCGCATGACGTTCGAATTCCAGGAC
GGCAACGCCTACATTCTTGATTATGAGGATTATCACTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10765.36 Da
• Isoelectric Point: 6.2137

>AT61739 WP_058090861.1 NZ_CP014754:2420557-2420850 [Marinobacter sp. LQ44]
MAMHNPPHPGEFIREVYLEPFGISSRQLASSLGVSPSTLSRLLKGDSGISPEMSLRLSKVLGRTPESWLAMQDMYDLWVA
RNKVNLDGIHPLDFEAA

Nucleotide

• Length: 294 bp

>AT61739 NZ_CP014754:2420557-2420850 [Marinobacter sp. LQ44]
ATGGCTATGCATAATCCGCCGCATCCGGGTGAATTCATTCGCGAGGTGTACCTTGAGCCTTTCGGTATCAGCTCCCGTCA
GCTTGCTTCCAGTCTGGGCGTATCACCTTCGACTTTGTCTCGGCTGCTCAAAGGAGATAGTGGTATTAGCCCGGAAATGT
CATTGCGGCTATCCAAGGTTCTCGGGCGCACTCCTGAGAGCTGGTTGGCTATGCAGGATATGTACGATCTTTGGGTGGCC
CGCAATAAGGTCAATCTGGATGGTATTCATCCCCTGGACTTCGAAGCAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A142FRV1

Antitoxin

• AlphaFold DB: A0A142FRV2