Detailed information of TA system TA061717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 127265..127917
• Replicon: plasmid unnamed
• Accession: NZ_CP014703
• Organism: Ralstonia solanacearum strain KACC 10722

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G3AAL2
• Locus tag: LBM2029_RS16840
• Protein ID: WP_075465943.1
• Coordinates: 127265..127609 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LBM2029_RS16845
• Protein ID: WP_075465945.1
• Coordinates: 127606..127917 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LBM2029_RS16825	122988..124151	-	1164	WP_075465937.1	2-methylcitrate synthase	-
LBM2029_RS16830	124186..125082	-	897	WP_082317449.1	methylisocitrate lyase	-
LBM2029_RS16835	125277..127214	+	1938	WP_075465941.1	propionate catabolism operon regulatory protein PrpR	-
LBM2029_RS16840	127265..127609	+	345	WP_075465943.1	toxin	Toxin
LBM2029_RS16845	127606..127917	+	312	WP_075465945.1	transcriptional regulator	Antitoxin
LBM2029_RS16850	128057..128884	-	828	WP_075468526.1	DUF2242 domain-containing protein	-
LBM2029_RS16855	129209..129574	+	366	WP_075468528.1	hypothetical protein	-
LBM2029_RS16860	129594..130193	+	600	WP_157771967.1	hypothetical protein	-
LBM2029_RS16865	130215..130646	-	432	WP_075465948.1	hypothetical protein	-
LBM2029_RS16870	130676..131008	-	333	WP_075465950.1	DUF5076 domain-containing protein	-
LBM2029_RS16875	131664..132026	-	363	WP_075465952.1	hypothetical protein	-
LBM2029_RS16880	132381..132863	-	483	WP_157771968.1	YcxB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1883432	1883432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13434.09 Da
• Isoelectric Point: 5.9519

>T61717 WP_075465943.1 NZ_CP014703:127265-127609 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLAQPDAGDVIRGTSGLRKLRFSDKRRGKGKRGGLRVIYYYWDEGGQFWM
FAVYDKDEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T61717 NZ_CP014703:127265-127609 [Ralstonia solanacearum]
ATGGATGCTACCTTTATTGAGTTGCCGCCTTTCCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACAGAGCACT
TCAACAGGAACTGCTTGCCCAGCCGGACGCCGGCGACGTCATCAGGGGGACAAGTGGTCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTCATTTACTACTACTGGGATGAAGGCGGCCAGTTCTGGATG
TTTGCCGTCTATGACAAGGACGAGGCGGACGACCTCTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11575.53 Da
• Isoelectric Point: 10.7132

>AT61717 WP_075465945.1 NZ_CP014703:127606-127917 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKTTLRKVKVRVLQPVDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT61717 NZ_CP014703:127606-127917 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGTGAAGGCAAGACGACCCTTCG
CAAGGTCAAGGTCCGCGTGCTCCAGCCGGTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCCT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGTACCTACCAGAATTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCAGTGTTGATCAAGCTCGTGGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: G3AAL2