Detailed information of TA system TA061696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4996173..4996828
• Replicon: chromosome
• Accession: NZ_CP014693
• Organism: Pseudomonas brassicacearum strain L13-6-12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G8QAX9
• Locus tag: A0U95_RS21790
• Protein ID: WP_013694185.1
• Coordinates: 4996173..4996517 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A0U95_RS21795
• Protein ID: WP_003205391.1
• Coordinates: 4996514..4996828 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0U95_RS21765	4991187..4992134	+	948	WP_003205397.1	ubiquinol oxidase subunit II	-
A0U95_RS21770	4992138..4994168	+	2031	WP_069971400.1	cytochrome o ubiquinol oxidase subunit I	-
A0U95_RS21775	4994172..4994798	+	627	WP_003205395.1	cytochrome o ubiquinol oxidase subunit III	-
A0U95_RS21780	4994799..4995134	+	336	WP_003205394.1	cytochrome o ubiquinol oxidase subunit IV	-
A0U95_RS21785	4995146..4996033	+	888	WP_003205393.1	heme o synthase	-
A0U95_RS21790	4996173..4996517	+	345	WP_013694185.1	hypothetical protein	Toxin
A0U95_RS21795	4996514..4996828	+	315	WP_003205391.1	helix-turn-helix domain-containing protein	Antitoxin
A0U95_RS21800	4997041..4997745	-	705	WP_003205390.1	hypothetical protein	-
A0U95_RS21805	4997867..4998610	-	744	WP_013694184.1	DUF72 domain-containing protein	-
A0U95_RS21810	4998702..4999715	-	1014	WP_003205388.1	diguanylate cyclase	-
A0U95_RS21815	4999865..5000782	-	918	WP_069971401.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-87)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13753.72 Da
• Isoelectric Point: 8.3860

>T61696 WP_013694185.1 NZ_CP014693:4996173-4996517 [Pseudomonas brassicacearum]
MRTLFFETTTFTDTVGHYLTDDEYRLLQSYMLQHPEVGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDRQFWM
FAIYDKDELENLTSEQEKTLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T61696 NZ_CP014693:4996173-4996517 [Pseudomonas brassicacearum]
ATGCGTACCCTATTTTTTGAAACGACTACATTCACCGACACGGTTGGCCATTACCTGACTGATGATGAGTATCGCCTGCT
TCAGTCCTATATGCTGCAGCATCCAGAGGTTGGTGACGTCATGCCACGTACCGGCGGCTTTCGTAAGCTGCGCTGGTTCG
ATGAACGTCGTGGTAAAGGGAAGCGAGGCGGGTTGCGCGTTATTTACTATTGGCTCATGAACGATCGTCAGTTCTGGATG
TTCGCGATTTATGACAAGGACGAGTTGGAAAACCTGACCTCCGAGCAAGAGAAGACGCTCAAACGCGCCATAGAAGCTGA
GTTGAAAGTACGAGGTACCTTATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.76 Da
• Isoelectric Point: 9.8409

>AT61696 WP_003205391.1 NZ_CP014693:4996514-4996828 [Pseudomonas brassicacearum]
MKKRDLFAEMMQGVEEMAAHREGKITLRQISIEDKPVPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSKP
NAQAALLIKLVEKFPDMVERLGAV

Nucleotide

• Length: 315 bp

>AT61696 NZ_CP014693:4996514-4996828 [Pseudomonas brassicacearum]
ATGAAAAAGCGCGATCTGTTTGCCGAGATGATGCAAGGCGTCGAGGAGATGGCCGCCCACCGGGAGGGCAAGATTACCCT
TCGCCAGATATCGATCGAAGACAAACCGGTTCCCGAGGTGTCTGCCCAAGAAATCGTGGCGTTGCGCGACAAGCTCCACA
TGTCTCAAGCCGTTTTCGCCAAGAGCATCAGGACCAGTCCAGGTACGCTCAGGAACTGGGAGCAGGAGAAGTCCAAGCCT
AACGCCCAGGCGGCTTTGTTGATCAAGCTGGTCGAGAAGTTTCCAGATATGGTCGAACGGCTTGGCGCTGTTTGA

Structures

Toxin

• AlphaFold DB: G8QAX9