Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 135506..136077 Replicon chromosome
Accession NZ_CP014671
Organism Immundisolibacter cernigliae strain TR3.2

Toxin (Protein)


Gene name higB Uniprot ID A0A1B1YQ18
Locus tag PG2T_RS00690 Protein ID WP_068802369.1
Coordinates 135506..135784 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A1B1YQ12
Locus tag PG2T_RS00695 Protein ID WP_068802370.1
Coordinates 135784..136077 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PG2T_RS00665 130954..131823 + 870 WP_068802365.1 xanthine dehydrogenase family protein subunit M -
PG2T_RS00670 131857..132321 + 465 WP_068802366.1 SRPBCC family protein -
PG2T_RS00675 132377..133387 + 1011 WP_068802367.1 ketopantoate reductase family protein -
PG2T_RS00680 134173..134709 + 537 WP_158513110.1 hypothetical protein -
PG2T_RS00685 135225..135401 - 177 WP_158513111.1 hypothetical protein -
PG2T_RS00690 135506..135784 + 279 WP_068802369.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PG2T_RS00695 135784..136077 + 294 WP_068802370.1 HigA family addiction module antidote protein Antitoxin
PG2T_RS00700 136833..137390 + 558 WP_068802371.1 antibiotic biosynthesis monooxygenase -
PG2T_RS00705 137587..138204 + 618 WP_068802372.1 class I SAM-dependent methyltransferase -
PG2T_RS00710 138343..138954 + 612 WP_068802373.1 hypothetical protein -
PG2T_RS00715 138997..140586 - 1590 WP_068802374.1 AMP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(3-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10511.94 Da        Isoelectric Point: 8.5042

>T61678 WP_068802369.1 NZ_CP014671:135506-135784 [Immundisolibacter cernigliae]
MIKSLKHKGLEEFFNSGSTRGIQAAHANKLRMQLAALDTATVIEDMNIPGYRLHPLKGDRKGLWSITVNGNWRVTFEFTD
GNVYIVNYEDYH

Download         Length: 279 bp

>T61678 NZ_CP014671:135506-135784 [Immundisolibacter cernigliae]
ATGATCAAGTCACTCAAGCATAAGGGGCTGGAGGAGTTTTTTAACTCCGGCTCTACTCGCGGTATTCAGGCGGCACATGC
CAATAAACTGCGTATGCAGCTGGCCGCTTTGGATACCGCTACAGTCATAGAAGACATGAACATCCCCGGCTACCGCCTGC
ACCCGCTGAAAGGCGATCGCAAGGGGCTTTGGTCAATTACGGTCAACGGGAACTGGCGCGTCACGTTCGAGTTCACGGAC
GGCAATGTCTATATCGTCAATTACGAGGACTACCACTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10720.42 Da        Isoelectric Point: 8.0648

>AT61678 WP_068802370.1 NZ_CP014671:135784-136077 [Immundisolibacter cernigliae]
MAMHNPPHPGEFILATYMEPFGVSCRSLAEHLDVAASTLNRILKQQSGVSPEMALRLSKVLGRSPESWLAMQDAYDLWVA
RKTVKLSGVQRLNLDAA

Download         Length: 294 bp

>AT61678 NZ_CP014671:135784-136077 [Immundisolibacter cernigliae]
ATGGCAATGCACAATCCTCCCCATCCCGGCGAGTTCATCCTGGCGACGTACATGGAGCCTTTTGGGGTCAGCTGCCGGTC
TCTTGCCGAACACCTTGATGTGGCTGCTTCCACCCTTAATCGGATACTGAAGCAGCAGAGTGGTGTTAGCCCGGAAATGG
CGCTGCGTCTCTCGAAGGTGCTCGGCCGCTCCCCTGAGAGCTGGCTTGCCATGCAGGACGCTTATGACCTTTGGGTGGCC
AGGAAAACGGTCAAGCTGTCTGGTGTGCAGCGTCTCAATCTCGATGCCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B1YQ18

Antitoxin

Source ID Structure
AlphaFold DB A0A1B1YQ12

References