Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4398068..4398670 Replicon chromosome
Accession NZ_CP014667
Organism Escherichia coli strain ECONIH2

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag WM48_RS21535 Protein ID WP_000897305.1
Coordinates 4398068..4398379 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag WM48_RS21540 Protein ID WP_000356397.1
Coordinates 4398380..4398670 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM48_RS21510 4393982..4394581 + 600 WP_001298594.1 glucose-1-phosphatase -
WM48_RS21515 4394575..4395447 + 873 WP_000920762.1 virulence factor BrkB family protein -
WM48_RS21520 4395444..4395881 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
WM48_RS21525 4395926..4396867 + 942 Protein_4112 fatty acid biosynthesis protein FabY -
WM48_RS21530 4396931..4397839 - 909 WP_001298596.1 alpha/beta hydrolase -
WM48_RS21535 4398068..4398379 + 312 WP_000897305.1 hypothetical protein Toxin
WM48_RS21540 4398380..4398670 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
WM48_RS21550 4399275..4399493 + 219 WP_001298592.1 ribbon-helix-helix domain-containing protein -
WM48_RS21555 4399708..4400637 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
WM48_RS21560 4400634..4401269 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
WM48_RS21565 4401266..4402168 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12172.16 Da        Isoelectric Point: 9.7791

>T61669 WP_000897305.1 NZ_CP014667:4398068-4398379 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T61669 NZ_CP014667:4398068-4398379 [Escherichia coli]
ATCCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT61669 WP_000356397.1 NZ_CP014667:4398380-4398670 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT61669 NZ_CP014667:4398380-4398670 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References