Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4085906..4086447 Replicon chromosome
Accession NZ_CP014642
Organism Escherichia coli strain BLK16

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag AYR48_RS20710 Protein ID WP_000615981.1
Coordinates 4085906..4086184 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AYR48_RS20715 Protein ID WP_000729704.1
Coordinates 4086187..4086447 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR48_RS20695 (4083488) 4083488..4084066 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AYR48_RS20700 (4084272) 4084272..4085039 + 768 WP_000333379.1 class II glutamine amidotransferase -
AYR48_RS20705 (4085010) 4085010..4085750 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AYR48_RS20710 (4085906) 4085906..4086184 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AYR48_RS20715 (4086187) 4086187..4086447 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AYR48_RS20720 (4086633) 4086633..4087406 + 774 WP_001353765.1 C40 family peptidase -
AYR48_RS20725 (4087582) 4087582..4088079 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
AYR48_RS20730 (4088295) 4088295..4090007 - 1713 Protein_3792 flagellar biosynthesis protein FlhA -
AYR48_RS20735 (4089979) 4089979..4090764 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC 4073107..4130370 57263


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T61617 WP_000615981.1 NZ_CP014642:c4086184-4085906 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T61617 NZ_CP014642:c4086184-4085906 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT61617 WP_000729704.1 NZ_CP014642:c4086447-4086187 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT61617 NZ_CP014642:c4086447-4086187 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References