Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4085717..4086258 Replicon chromosome
Accession NZ_CP014641
Organism Escherichia coli strain BLK9

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag AYL54_RS20695 Protein ID WP_000615981.1
Coordinates 4085717..4085995 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AYL54_RS20700 Protein ID WP_000729704.1
Coordinates 4085998..4086258 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYL54_RS20680 (4083299) 4083299..4083877 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AYL54_RS20685 (4084083) 4084083..4084850 + 768 WP_000333379.1 class II glutamine amidotransferase -
AYL54_RS20690 (4084821) 4084821..4085561 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AYL54_RS20695 (4085717) 4085717..4085995 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AYL54_RS20700 (4085998) 4085998..4086258 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AYL54_RS20705 (4086444) 4086444..4087217 + 774 WP_001353765.1 C40 family peptidase -
AYL54_RS20710 (4087393) 4087393..4087890 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
AYL54_RS20715 (4088106) 4088106..4089818 - 1713 Protein_3806 flagellar biosynthesis protein FlhA -
AYL54_RS20720 (4089790) 4089790..4090575 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - IlpA / gmhA/lpcA 4043151..4098729 55578


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T61597 WP_000615981.1 NZ_CP014641:c4085995-4085717 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T61597 NZ_CP014641:c4085995-4085717 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT61597 WP_000729704.1 NZ_CP014641:c4086258-4085998 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT61597 NZ_CP014641:c4086258-4085998 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References