Detailed information of TA system TA061572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1537932..1538547
• Replicon: chromosome
• Accession: NZ_CP014636
• Organism: Vibrio vulnificus strain CECT 4999

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: VVCECT4999_RS07585
• Protein ID: WP_001162670.1
• Coordinates: 1537932..1538219 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: VVCECT4999_RS07590
• Protein ID: WP_001232701.1
• Coordinates: 1538230..1538547 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VVCECT4999_RS07535	1533441..1534337	+	897	WP_039452295.1	hypothetical protein	-
VVCECT4999_RS07540	1534321..1534455	+	135	WP_080729748.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07550	1534816..1534944	+	129	WP_080729749.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07555	1535440..1535727	+	288	WP_039452301.1	hypothetical protein	-
VVCECT4999_RS07560	1535887..1536432	+	546	WP_039452304.1	GNAT family N-acetyltransferase	-
VVCECT4999_RS07565	1536577..1537044	+	468	WP_039452307.1	hypothetical protein	-
VVCECT4999_RS07570	1537081..1537185	+	105	WP_080729747.1	hypothetical protein	-
VVCECT4999_RS07575	1537222..1537713	+	492	WP_039452310.1	hypothetical protein	-
VVCECT4999_RS07585	1537932..1538219	+	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
VVCECT4999_RS07590	1538230..1538547	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
VVCECT4999_RS24770	1538544..1538654	+	111	WP_082798255.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07605	1539023..1539151	+	129	WP_080729750.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07610	1539495..1539638	+	144	WP_080729751.1	DUF3265 domain-containing protein	-
VVCECT4999_RS07615	1539643..1540395	+	753	WP_039452354.1	hypothetical protein	-
VVCECT4999_RS07620	1540536..1541567	+	1032	WP_039452357.1	hypothetical protein	-
VVCECT4999_RS07625	1541715..1542179	+	465	WP_038964939.1	ASCH domain-containing protein	-
VVCECT4999_RS07635	1542872..1543405	+	534	WP_088900024.1	cysteine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1453300..1639357	186057
	-	inside	Integron	-	-	1531219..1607550	76331

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T61572 WP_001162670.1 NZ_CP014636:1537932-1538219 [Vibrio vulnificus]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T61572 NZ_CP014636:1537932-1538219 [Vibrio vulnificus]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAATGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT61572 WP_001232701.1 NZ_CP014636:1538230-1538547 [Vibrio vulnificus]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT61572 NZ_CP014636:1538230-1538547 [Vibrio vulnificus]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTGAGTAATTTGATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCAGCGGCATTAGGCAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36