Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4910426..4910928 Replicon chromosome
Accession NZ_CP014583
Organism Escherichia coli strain CFSAN004176

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BCV59_RS26055 Protein ID WP_000767829.1
Coordinates 4910674..4910928 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BCV59_RS26050 Protein ID WP_001259255.1
Coordinates 4910426..4910677 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCV59_RS26030 4905604..4906671 - 1068 WP_069357614.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BCV59_RS26035 4906671..4907741 - 1071 WP_000108944.1 histidinol-phosphate transaminase -
BCV59_RS26040 4907738..4909042 - 1305 WP_000009587.1 histidinol dehydrogenase -
BCV59_RS26045 4909048..4909947 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BCV59_RS29700 4910093..4910143 - 51 WP_001364200.1 his operon leader peptide -
BCV59_RS26050 4910426..4910677 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BCV59_RS26055 4910674..4910928 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BCV59_RS26060 4911011..4911835 + 825 WP_000754737.1 SDR family oxidoreductase -
BCV59_RS26065 4911881..4912810 + 930 WP_000803351.1 LysR family transcriptional regulator -
BCV59_RS31350 4913025..4913087 + 63 WP_010723108.1 membrane protein YoeI -
BCV59_RS26070 4913077..4914435 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
BCV59_RS26075 4914614..4915672 + 1059 WP_000492305.1 transport protein YeeE -
BCV59_RS26080 4915686..4915913 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T61542 WP_000767829.1 NZ_CP014583:4910674-4910928 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T61542 NZ_CP014583:4910674-4910928 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT61542 WP_001259255.1 NZ_CP014583:4910426-4910677 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT61542 NZ_CP014583:4910426-4910677 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References