Detailed information of TA system TA061540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4104083..4104810
• Replicon: chromosome
• Accession: NZ_CP014583
• Organism: Escherichia coli strain CFSAN004176

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: BCV59_RS22035
• Protein ID: WP_000547563.1
• Coordinates: 4104083..4104394 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BCV59_RS22040
• Protein ID: WP_000126296.1
• Coordinates: 4104391..4104810 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCV59_RS22005	4099226..4100935	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
BCV59_RS22010	4100945..4101487	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
BCV59_RS22015	4101487..4102254	+	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
BCV59_RS22020	4102251..4102661	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
BCV59_RS22025	4102654..4103124	+	471	WP_069357505.1	hydrogenase maturation peptidase HycI	-
BCV59_RS22030	4103149..4103921	+	773	Protein_3948	hypothetical protein	-
BCV59_RS22035	4104083..4104394	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
BCV59_RS22040	4104391..4104810	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
BCV59_RS22045	4104924..4106348	-	1425	WP_024226292.1	6-phospho-beta-glucosidase AscB	-
BCV59_RS22050	4106357..4107814	-	1458	Protein_3952	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
BCV59_RS22055	4108074..4109084	+	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
BCV59_RS22060	4109233..4109760	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T61540 WP_000547563.1 NZ_CP014583:4104083-4104394 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T61540 NZ_CP014583:4104083-4104394 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT61540 WP_000126296.1 NZ_CP014583:4104391-4104810 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT61540 NZ_CP014583:4104391-4104810 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22