Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1766370..1766911 Replicon chromosome
Accession NZ_CP014583
Organism Escherichia coli strain CFSAN004176

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag BCV59_RS10360 Protein ID WP_000615979.1
Coordinates 1766633..1766911 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag BCV59_RS10355 Protein ID WP_000729704.1
Coordinates 1766370..1766630 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCV59_RS10325 1761399..1762454 - 1056 WP_001226164.1 DNA polymerase IV -
BCV59_RS10330 1762525..1763310 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
BCV59_RS10335 1763282..1764622 + 1341 Protein_1748 flagellar biosynthesis protein FlhA -
BCV59_RS10340 1764727..1765005 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
BCV59_RS10345 1764998..1765354 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
BCV59_RS10350 1765411..1766184 - 774 WP_000543899.1 C40 family peptidase -
BCV59_RS10355 1766370..1766630 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
BCV59_RS10360 1766633..1766911 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
BCV59_RS10365 1767067..1767807 + 741 WP_001225679.1 murein L,D-transpeptidase -
BCV59_RS10370 1767778..1768545 - 768 WP_001365365.1 class II glutamine amidotransferase -
BCV59_RS10375 1768751..1769329 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T61531 WP_000615979.1 NZ_CP014583:1766633-1766911 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T61531 NZ_CP014583:1766633-1766911 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT61531 WP_000729704.1 NZ_CP014583:1766370-1766630 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT61531 NZ_CP014583:1766370-1766630 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References