Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3526179..3526932 Replicon chromosome
Accession NZ_CP014578
Organism Paraburkholderia phytofirmans OLGA172

Toxin (Protein)


Gene name graT Uniprot ID A0A167W2I8
Locus tag AYM40_RS15440 Protein ID WP_063496971.1
Coordinates 3526179..3526460 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A160FME1
Locus tag AYM40_RS15445 Protein ID WP_063496972.1
Coordinates 3526477..3526932 (+) Length 152 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYM40_RS15430 3522369..3523067 - 699 WP_063496969.1 DUF4136 domain-containing protein -
AYM40_RS15435 3523284..3525980 + 2697 WP_063496970.1 aminopeptidase N -
AYM40_RS15440 3526179..3526460 + 282 WP_063496971.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AYM40_RS15445 3526477..3526932 + 456 WP_063496972.1 HigA family addiction module antidote protein Antitoxin
AYM40_RS15450 3527055..3528071 + 1017 WP_063496973.1 class 1 fructose-bisphosphatase -
AYM40_RS15460 3528408..3528608 - 201 WP_063496974.1 hypothetical protein -
AYM40_RS41925 3529599..3529748 + 150 WP_028200145.1 hypothetical protein -
AYM40_RS15465 3530039..3530635 + 597 WP_063496975.1 chorismate mutase -
AYM40_RS15470 3530749..3531903 - 1155 WP_063496976.1 DNA polymerase III subunit epsilon -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-93)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(23-116)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10539.12 Da        Isoelectric Point: 9.0918

>T61505 WP_063496971.1 NZ_CP014578:3526179-3526460 [Paraburkholderia phytofirmans OLGA172]
MITTFGDKATAAIFQGKFVHSLPLYMQALARRKLLMIDAAESSRNLHAPPGNRLEALQGQRSGQWSIRINAQWRICFHFV
DGEALNVEIVDYH

Download         Length: 282 bp

>T61505 NZ_CP014578:3526179-3526460 [Paraburkholderia phytofirmans OLGA172]
ATGATCACGACATTCGGCGACAAAGCTACGGCGGCAATCTTTCAGGGCAAGTTCGTGCATTCATTGCCGCTCTACATGCA
GGCGCTCGCACGCCGCAAGCTGTTGATGATCGATGCCGCTGAATCCAGCCGTAACCTGCATGCGCCGCCGGGCAACCGGC
TCGAAGCCTTGCAAGGTCAGCGTAGCGGACAGTGGAGTATTCGAATCAACGCGCAGTGGCGCATCTGTTTTCATTTTGTC
GACGGGGAGGCGCTGAATGTCGAAATTGTCGACTATCACTGA

Antitoxin


Download         Length: 152 a.a.        Molecular weight: 16464.10 Da        Isoelectric Point: 10.5174

>AT61505 WP_063496972.1 NZ_CP014578:3526477-3526932 [Paraburkholderia phytofirmans OLGA172]
MVIKRSDLGSIDFAGIDTGESIPEIHPGEILRSEFLEPLGMSVNALALALRVPAPRINDIVRGKRAISADTALRLERYFG
ASAQFWLNLQIAYDLRVATAAAGEQIEREIEPMPKANRPKMPKVSAEHARATALAARLTGHVTAIKARRKA

Download         Length: 456 bp

>AT61505 NZ_CP014578:3526477-3526932 [Paraburkholderia phytofirmans OLGA172]
ATGGTCATCAAACGCTCCGATCTGGGCAGCATCGATTTCGCCGGCATCGACACCGGCGAAAGCATCCCGGAAATCCACCC
CGGCGAGATTCTGCGCAGCGAGTTCCTCGAACCGCTTGGCATGTCCGTCAATGCGCTCGCACTTGCGCTGCGCGTACCGG
CGCCGCGCATCAACGACATCGTGCGTGGCAAGCGCGCCATCTCGGCTGACACCGCGTTGCGGCTCGAGCGCTACTTCGGT
GCGAGCGCGCAGTTCTGGCTGAATCTGCAGATCGCCTACGACCTGCGCGTCGCCACCGCGGCAGCCGGCGAGCAGATCGA
ACGCGAGATCGAGCCGATGCCCAAGGCGAACCGGCCGAAAATGCCGAAAGTCTCAGCCGAACACGCCCGTGCCACAGCGC
TTGCCGCCCGCCTCACCGGCCATGTCACGGCCATCAAAGCTCGCCGCAAAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A167W2I8

Antitoxin

Source ID Structure
AlphaFold DB A0A160FME1

References