Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2185868..2186736 Replicon chromosome
Accession NZ_CP014566
Organism Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag AZH48_RS10130 Protein ID WP_010886136.1
Coordinates 2185868..2186245 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag AZH48_RS10135 Protein ID WP_003409886.1
Coordinates 2186287..2186736 (+) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZH48_RS10080 2181657..2182109 - 453 WP_010950636.1 lipoprotein -
AZH48_RS10085 2182173..2182574 + 402 WP_003409869.1 hypothetical protein -
AZH48_RS10090 2182567..2182749 - 183 WP_003409870.1 hypothetical protein -
AZH48_RS10095 2182863..2183213 - 351 WP_003409871.1 hypothetical protein -
AZH48_RS10100 2183224..2184126 - 903 WP_003409874.1 hypothetical protein -
AZH48_RS10105 2184147..2184338 - 192 WP_003409876.1 hypothetical protein -
AZH48_RS10110 2184339..2184635 - 297 WP_003409877.1 hypothetical protein -
AZH48_RS10115 2184875..2185090 + 216 WP_003409878.1 antitoxin -
AZH48_RS10120 2185087..2185398 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
AZH48_RS22255 2185372..2185893 - 522 WP_010950637.1 hypothetical protein -
AZH48_RS10130 2185868..2186245 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
AZH48_RS10135 2186287..2186736 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
AZH48_RS10140 2186733..2187278 + 546 WP_003409891.1 SecB-like chaperone -
AZH48_RS10145 2187167..2187781 - 615 WP_003901296.1 hypothetical protein -
AZH48_RS10150 2187830..2188126 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
AZH48_RS10155 2188123..2188374 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
AZH48_RS10160 2188361..2188855 + 495 WP_003899099.1 hypothetical protein -
AZH48_RS10165 2189015..2189422 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
AZH48_RS10170 2189426..2189698 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
AZH48_RS10175 2189731..2190951 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(27-113)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-91)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T61471 WP_010886136.1 NZ_CP014566:2185868-2186245 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T61471 NZ_CP014566:2185868-2186245 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT61471 WP_003409886.1 NZ_CP014566:2186287-2186736 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT61471 NZ_CP014566:2186287-2186736 [Mycobacterium tuberculosis variant bovis BCG str. Tokyo 172]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU

Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References