Detailed information of TA system TA061436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4057693..4058356
• Replicon: chromosome
• Accession: NZ_CP014546
• Organism: Pseudomonas azotoformans strain S4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AYR47_RS32190
• Protein ID: WP_082781571.1
• Coordinates: 4057693..4058058 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W2DIY0
• Locus tag: AYR47_RS18955
• Protein ID: WP_033901597.1
• Coordinates: 4058051..4058356 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR47_RS18925	4052915..4053469	+	555	WP_061436306.1	histidine phosphatase family protein	-
AYR47_RS18930	4053515..4054270	+	756	WP_033901602.1	precorrin-6A synthase (deacetylating)	-
AYR47_RS18935	4054282..4055001	+	720	WP_033901601.1	dienelactone hydrolase family protein	-
AYR47_RS18940	4055013..4055567	-	555	WP_033901600.1	hypothetical protein	-
AYR47_RS18945	4055640..4057449	-	1810	Protein_3761	cation:proton antiporter	-
AYR47_RS32190	4057693..4058058	+	366	WP_082781571.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYR47_RS18955	4058051..4058356	+	306	WP_033901597.1	XRE family transcriptional regulator	Antitoxin
AYR47_RS18960	4058407..4059285	+	879	WP_061436308.1	LysR family transcriptional regulator	-
AYR47_RS18965	4059275..4059676	-	402	WP_061436309.1	hypothetical protein	-
AYR47_RS18970	4059759..4060661	-	903	WP_033901593.1	EamA family transporter	-
AYR47_RS18975	4060715..4062619	-	1905	WP_061436310.1	asparagine synthase (glutamine-hydrolyzing)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (17-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13852.60 Da
• Isoelectric Point: 6.8450

>T61436 WP_082781571.1 NZ_CP014546:4057693-4058058 [Pseudomonas azotoformans]
MSWDVEYTDEFGDWWDDLGTKEQISVSASVDLLGLLGPGLGFPHSSDIKGSRHGNLRELRIQHAGRPFRVLYAFDPRRCA
VLLIGGDKTGRHRWYEEHVPLAEKLYDVHLDTLRKEGRKHG

Nucleotide

• Length: 366 bp

>T61436 NZ_CP014546:4057693-4058058 [Pseudomonas azotoformans]
ATGAGCTGGGACGTTGAATACACGGATGAATTCGGAGACTGGTGGGATGACTTGGGAACAAAGGAACAAATTTCCGTTTC
CGCCAGCGTTGATTTACTGGGGCTTTTAGGGCCCGGATTGGGCTTTCCCCACAGCAGCGATATCAAGGGATCCCGGCATG
GCAACCTGCGGGAACTGCGCATACAGCATGCAGGGCGGCCATTTCGAGTGTTGTATGCCTTTGATCCACGACGATGCGCG
GTGTTGCTGATCGGTGGTGACAAGACAGGACGGCATCGATGGTATGAGGAACACGTCCCGTTGGCTGAAAAGCTGTATGA
CGTGCATTTGGACACTTTGCGTAAAGAGGGCCGTAAACATGGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11570.41 Da
• Isoelectric Point: 9.5353

>AT61436 WP_033901597.1 NZ_CP014546:4058051-4058356 [Pseudomonas azotoformans]
MAKKFAELQARMTPESRAEAKLLFQQHLQEMPLHELRKAQQLSQASLAKALNINQAAVSKMERRTDMYISTLRDYIRAMG
GELEIIATFPDGQVKIENFAY

Nucleotide

• Length: 306 bp

>AT61436 NZ_CP014546:4058051-4058356 [Pseudomonas azotoformans]
ATGGCTAAGAAATTCGCTGAACTGCAAGCGCGCATGACACCGGAGTCCCGTGCCGAAGCCAAGCTGTTGTTCCAGCAGCA
CCTGCAGGAAATGCCACTGCATGAGTTGCGCAAAGCTCAGCAGCTAAGCCAGGCGAGTCTGGCGAAAGCACTGAACATCA
ACCAGGCGGCCGTCTCCAAGATGGAGCGGCGAACCGACATGTACATCAGCACGTTGCGCGACTATATCCGCGCGATGGGC
GGCGAGTTGGAGATTATTGCGACGTTTCCCGATGGCCAGGTCAAGATCGAGAACTTTGCTTACTGA

Structures

Antitoxin

• AlphaFold DB: W2DIY0