Detailed information of TA system TA061435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 3141951..3142555
• Replicon: chromosome
• Accession: NZ_CP014546
• Organism: Pseudomonas azotoformans strain S4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W2D682
• Locus tag: AYR47_RS14625
• Protein ID: WP_033902644.1
• Coordinates: 3142241..3142555 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AYR47_RS14620
• Protein ID: WP_016975178.1
• Coordinates: 3141951..3142238 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR47_RS14590	3137590..3138468	+	879	WP_033902649.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AYR47_RS14595	3138486..3139601	+	1116	WP_028619346.1	outer membrane protein assembly factor BamC	-
AYR47_RS14600	3139606..3140364	+	759	WP_033902647.1	MBL fold metallo-hydrolase	-
AYR47_RS14605	3140392..3141105	+	714	WP_033902646.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AYR47_RS14620	3141951..3142238	-	288	WP_016975178.1	helix-turn-helix domain-containing protein	Antitoxin
AYR47_RS14625	3142241..3142555	-	315	WP_033902644.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYR47_RS32070	3142707..3142964	+	258	Protein_2910	DUF86 domain-containing protein	-
AYR47_RS14630	3143022..3144248	-	1227	WP_061435677.1	MFS transporter	-
AYR47_RS14635	3144531..3144779	+	249	WP_033902642.1	hypothetical protein	-
AYR47_RS14640	3144847..3145785	-	939	WP_061435679.1	LysR family transcriptional regulator	-
AYR47_RS14645	3145944..3147263	+	1320	WP_016975188.1	MHS family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11735.56 Da
• Isoelectric Point: 9.7805

>T61435 WP_033902644.1 NZ_CP014546:c3142555-3142241 [Pseudomonas azotoformans]
MIFIETSGFTHRVKELIDDDAYMAFQNVLIVNPSAGDVIEGTGGVRKIRIAAKGHGKRGGARVIYYHFVSASKIVFLMIY
PKNEQQDLTADERKSLKVAIEHWR

Nucleotide

• Length: 315 bp

>T61435 NZ_CP014546:c3142555-3142241 [Pseudomonas azotoformans]
ATGATTTTCATCGAGACTTCAGGTTTCACCCATCGTGTCAAAGAACTGATCGACGACGATGCCTATATGGCGTTTCAAAA
TGTGCTGATCGTGAATCCATCAGCCGGTGATGTAATTGAAGGCACAGGCGGAGTGCGCAAGATTCGGATTGCGGCCAAGG
GCCACGGCAAACGAGGTGGAGCGCGGGTGATTTACTACCACTTTGTCTCGGCATCAAAGATCGTTTTTTTGATGATCTAC
CCTAAGAATGAACAGCAAGACCTTACCGCTGACGAGCGTAAATCGTTGAAGGTTGCAATCGAACACTGGAGATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10741.33 Da
• Isoelectric Point: 6.7288

>AT61435 WP_016975178.1 NZ_CP014546:c3142238-3141951 [Pseudomonas azotoformans]
MSKFFDELMESVEEMNEILRGEREPSREFHVDAMRVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRRDPTGPAKALLK
AIHNDPVHVLNALAG

Nucleotide

• Length: 288 bp

>AT61435 NZ_CP014546:c3142238-3141951 [Pseudomonas azotoformans]
ATGAGCAAATTTTTTGATGAACTGATGGAAAGCGTTGAGGAAATGAACGAGATACTGCGCGGTGAGCGTGAGCCTTCCCG
CGAGTTTCACGTTGACGCAATGCGGGTCAAAGAAATTCGTAAGGCGACTGGGCTGACCCAAGCTAAATTCGCAGCGTTGA
TTGATGTTCAACTGGGTACGCTGCGTAACTGGGAGCAAGGTCGCCGAGACCCAACGGGGCCAGCTAAGGCGTTGCTGAAG
GCGATCCACAATGACCCGGTGCATGTGCTGAACGCATTGGCCGGTTGA

Structures

Toxin

• AlphaFold DB: W2D682