Detailed information of TA system TA061397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4416619..4417221
• Replicon: chromosome
• Accession: NZ_CP014522
• Organism: Escherichia coli strain ZH063

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AVR76_RS22170
• Protein ID: WP_000897302.1
• Coordinates: 4416619..4416930 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AVR76_RS22175
• Protein ID: WP_000356397.1
• Coordinates: 4416931..4417221 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR76_RS22145	4412533..4413132	+	600	WP_001296610.1	glucose-1-phosphatase	-
AVR76_RS22150	4413126..4413998	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AVR76_RS22155	4413995..4414432	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AVR76_RS22160	4414477..4415418	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AVR76_RS22165	4415482..4416390	-	909	WP_001385591.1	alpha/beta hydrolase	-
AVR76_RS22170	4416619..4416930	+	312	WP_000897302.1	hypothetical protein	Toxin
AVR76_RS22175	4416931..4417221	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AVR76_RS22185	4417580..4417858	+	279	WP_001296612.1	hypothetical protein	-
AVR76_RS22190	4418255..4418473	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
AVR76_RS22195	4418658..4419107	-	450	WP_032140890.1	hypothetical protein	-
AVR76_RS22200	4419423..4420270	-	848	Protein_4229	hypothetical protein	-
AVR76_RS22205	4420560..4420802	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
AVR76_RS22210	4420984..4421913	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T61397 WP_000897302.1 NZ_CP014522:4416619-4416930 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T61397 NZ_CP014522:4416619-4416930 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT61397 WP_000356397.1 NZ_CP014522:4416931-4417221 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT61397 NZ_CP014522:4416931-4417221 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416