Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3546207..3547006 Replicon chromosome
Accession NZ_CP014522
Organism Escherichia coli strain ZH063

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag AVR76_RS17855 Protein ID WP_000347251.1
Coordinates 3546542..3547006 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag AVR76_RS17850 Protein ID WP_001296435.1
Coordinates 3546207..3546542 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR76_RS17835 3541992..3542762 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
AVR76_RS17840 3542778..3544112 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
AVR76_RS17845 3544487..3546058 + 1572 WP_001273763.1 galactarate dehydratase -
AVR76_RS17850 3546207..3546542 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AVR76_RS17855 3546542..3547006 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AVR76_RS17860 3547061..3547870 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
AVR76_RS17865 3548119..3549399 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
AVR76_RS17870 3549422..3549895 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
AVR76_RS17875 3549906..3550685 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
AVR76_RS17880 3550675..3551553 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
AVR76_RS17885 3551571..3552005 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T61394 WP_000347251.1 NZ_CP014522:3546542-3547006 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T61394 NZ_CP014522:3546542-3547006 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT61394 WP_001296435.1 NZ_CP014522:3546207-3546542 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT61394 NZ_CP014522:3546207-3546542 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References