Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3294527..3295175 Replicon chromosome
Accession NZ_CP014513
Organism Frondihabitans sp. PAMC 28766 strain SR6

Toxin (Protein)


Gene name graT Uniprot ID A0A126Z282
Locus tag AX769_RS15705 Protein ID WP_066281196.1
Coordinates 3294894..3295175 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A126Z294
Locus tag AX769_RS15700 Protein ID WP_066281195.1
Coordinates 3294527..3294823 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AX769_RS15680 3289556..3290062 - 507 WP_066281184.1 acetolactate synthase small subunit -
AX769_RS15685 3290059..3291921 - 1863 WP_082763860.1 acetolactate synthase large subunit -
AX769_RS15690 3291965..3293677 - 1713 WP_066281188.1 dihydroxy-acid dehydratase -
AX769_RS15695 3293688..3294461 - 774 WP_066281191.1 hypothetical protein -
AX769_RS15700 3294527..3294823 - 297 WP_066281195.1 HigA family addiction module antidote protein Antitoxin
AX769_RS15705 3294894..3295175 - 282 WP_066281196.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AX769_RS15710 3295276..3296073 - 798 WP_066281198.1 trehalose-phosphatase -
AX769_RS15715 3296070..3297584 - 1515 WP_066281200.1 trehalose-6-phosphate synthase -
AX769_RS15720 3297577..3298503 - 927 WP_082763861.1 fructosamine kinase family protein -
AX769_RS15725 3298502..3299470 + 969 WP_066281202.1 magnesium transporter CorA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-67)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10622.19 Da        Isoelectric Point: 10.2058

>T61368 WP_066281196.1 NZ_CP014513:c3295175-3294894 [Frondihabitans sp. PAMC 28766]
VIQTFADSATESVWLRRHAKRLSPELQRIAHRKLLMLDAAQSLVDLRIPPGNRLEALRGARAGQHSIRVNDQWRICFVWS
PTGPADVEIVDYH

Download         Length: 282 bp

>T61368 NZ_CP014513:c3295175-3294894 [Frondihabitans sp. PAMC 28766]
GTGATCCAGACGTTCGCCGACTCAGCGACCGAAAGCGTCTGGCTCCGACGGCACGCCAAGCGCCTCAGCCCCGAGCTGCA
GCGCATCGCCCACCGCAAACTGCTCATGCTCGACGCCGCCCAGTCGCTGGTCGACCTCCGGATTCCGCCCGGCAATCGAC
TCGAGGCCCTTCGGGGAGCACGCGCAGGGCAACACAGCATCCGCGTCAACGACCAGTGGCGCATCTGCTTCGTCTGGTCG
CCCACCGGCCCCGCCGACGTCGAGATCGTCGACTACCACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10925.53 Da        Isoelectric Point: 6.6435

>AT61368 WP_066281195.1 NZ_CP014513:c3294823-3294527 [Frondihabitans sp. PAMC 28766]
MSTMHAPIHPGEILLEEFLLPLGISQYRLSKAIEVPPRRINEIVHGTRGISTDTALRLARALGTSARFWLNLQTHFDLEA
ESEAHRAELDRITSLVGA

Download         Length: 297 bp

>AT61368 NZ_CP014513:c3294823-3294527 [Frondihabitans sp. PAMC 28766]
ATGTCGACGATGCACGCCCCCATCCACCCCGGGGAGATCCTGCTCGAAGAGTTCCTGCTGCCCCTCGGCATCAGCCAGTA
CCGCCTGTCCAAGGCCATCGAGGTGCCCCCGCGCCGCATCAACGAGATCGTCCACGGCACCCGCGGCATCTCGACCGACA
CGGCACTGCGGCTCGCTCGAGCGTTGGGTACGTCCGCCCGCTTCTGGCTCAATCTGCAGACGCACTTCGATCTCGAGGCA
GAGAGCGAAGCCCACCGCGCCGAGCTCGACCGGATCACCTCGCTCGTCGGGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A126Z282

Antitoxin

Source ID Structure
AlphaFold DB A0A126Z294

References