Detailed information of TA system TA061355

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 42694..42936
• Replicon: plasmid pZH193
• Accession: NZ_CP014498
• Organism: Escherichia coli strain ZH193

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AVR69_RS25495
• Protein ID: WP_001312861.1
• Coordinates: 42694..42852 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	42896..42936 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR69_RS25455	37806..38033	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
AVR69_RS25460	38121..38798	-	678	WP_001348626.1	PAS domain-containing protein	-
AVR69_RS25465	38932..39315	-	384	WP_001151566.1	relaxosome protein TraM	-
AVR69_RS25470	39646..40248	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
AVR69_RS25475	40545..41366	-	822	WP_001234469.1	DUF945 domain-containing protein	-
AVR69_RS25480	41485..41772	-	288	WP_000107535.1	hypothetical protein	-
AVR69_RS30305	41797..42003	-	207	WP_000275859.1	hypothetical protein	-
AVR69_RS25495	42694..42852	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	42896..42936	-	41	NuclAT_1	-	Antitoxin
-	42896..42936	-	41	NuclAT_1	-	Antitoxin
-	42896..42936	-	41	NuclAT_1	-	Antitoxin
-	42896..42936	-	41	NuclAT_1	-	Antitoxin
-	44380..44566	-	187	NuclAT_0	-	-
-	44380..44566	-	187	NuclAT_0	-	-
-	44380..44566	-	187	NuclAT_0	-	-
-	44380..44566	-	187	NuclAT_0	-	-
AVR69_RS25515	44578..45297	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
AVR69_RS25520	45294..45728	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
AVR69_RS25525	45783..47741	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / dfrA17 / aac(3)-IId / blaTEM-1B	senB	1..209768	209768

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T61355 WP_001312861.1 NZ_CP014498:c42852-42694 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T61355 NZ_CP014498:c42852-42694 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT61355 NZ_CP014498:c42936-42896 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin