Detailed information of TA system TA061351

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 858..1097
• Replicon: plasmid pZH193
• Accession: NZ_CP014498
• Organism: Escherichia coli strain ZH193

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: AVR69_RS25255
• Protein ID: WP_023144756.1
• Coordinates: 858..992 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	1037..1097 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR69_RS30300	67..201	-	135	Protein_2	protein CopA/IncA	-
AVR69_RS25250	307..561	-	255	WP_000083850.1	replication regulatory protein RepA	-
AVR69_RS25255	858..992	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	1037..1097	+	61	NuclAT_2	-	Antitoxin
-	1037..1097	+	61	NuclAT_2	-	Antitoxin
-	1037..1097	+	61	NuclAT_2	-	Antitoxin
-	1037..1097	+	61	NuclAT_2	-	Antitoxin
AVR69_RS25260	1064..1350	-	287	Protein_5	DUF2726 domain-containing protein	-
AVR69_RS25265	1428..3041	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
AVR69_RS25270	3072..3422	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
AVR69_RS25275	3419..3844	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
AVR69_RS25280	4403..4615	-	213	WP_013023861.1	hypothetical protein	-
AVR69_RS25285	4746..5306	-	561	WP_000139341.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / dfrA17 / aac(3)-IId / blaTEM-1B	senB	1..209768	209768

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T61351 WP_023144756.1 NZ_CP014498:c992-858 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T61351 NZ_CP014498:c992-858 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT61351 NZ_CP014498:1037-1097 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin