Detailed information of TA system TA061345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4394371..4394973
• Replicon: chromosome
• Accession: NZ_CP014497
• Organism: Escherichia coli strain ZH193

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AVR69_RS22025
• Protein ID: WP_000897302.1
• Coordinates: 4394371..4394682 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AVR69_RS22030
• Protein ID: WP_000356397.1
• Coordinates: 4394683..4394973 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR69_RS22000	4390285..4390884	+	600	WP_001296610.1	glucose-1-phosphatase	-
AVR69_RS22005	4390878..4391750	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AVR69_RS22010	4391747..4392184	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AVR69_RS22015	4392229..4393170	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AVR69_RS22020	4393234..4394142	-	909	WP_001385591.1	alpha/beta hydrolase	-
AVR69_RS22025	4394371..4394682	+	312	WP_000897302.1	hypothetical protein	Toxin
AVR69_RS22030	4394683..4394973	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AVR69_RS22040	4395332..4395610	+	279	WP_001296612.1	hypothetical protein	-
AVR69_RS22045	4396007..4396225	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
AVR69_RS22050	4396410..4396859	-	450	WP_032140890.1	hypothetical protein	-
AVR69_RS22055	4397175..4398023	-	849	WP_001038650.1	hypothetical protein	-
AVR69_RS22060	4398313..4398555	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
AVR69_RS22065	4398737..4399666	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T61345 WP_000897302.1 NZ_CP014497:4394371-4394682 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T61345 NZ_CP014497:4394371-4394682 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT61345 WP_000356397.1 NZ_CP014497:4394683-4394973 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT61345 NZ_CP014497:4394683-4394973 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416