Detailed information of TA system TA061343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3584369..3585168
• Replicon: chromosome
• Accession: NZ_CP014497
• Organism: Escherichia coli strain ZH193

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: AVR69_RS18005
• Protein ID: WP_000347251.1
• Coordinates: 3584704..3585168 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: AVR69_RS18000
• Protein ID: WP_001296435.1
• Coordinates: 3584369..3584704 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVR69_RS17985	3580154..3580924	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
AVR69_RS17990	3580940..3582274	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
AVR69_RS17995	3582649..3584220	+	1572	WP_001273763.1	galactarate dehydratase	-
AVR69_RS18000	3584369..3584704	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
AVR69_RS18005	3584704..3585168	+	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
AVR69_RS18010	3585223..3586032	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
AVR69_RS18015	3586281..3587561	+	1281	WP_000681950.1	tagatose-bisphosphate aldolase subunit KbaZ	-
AVR69_RS18020	3587584..3588057	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
AVR69_RS18025	3588068..3588847	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
AVR69_RS18030	3588837..3589715	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
AVR69_RS18035	3589733..3590167	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T61343 WP_000347251.1 NZ_CP014497:3584704-3585168 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T61343 NZ_CP014497:3584704-3585168 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT61343 WP_001296435.1 NZ_CP014497:3584369-3584704 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT61343 NZ_CP014497:3584369-3584704 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: S1PPV5