Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2090399..2090901 Replicon chromosome
Accession NZ_CP014488
Organism Escherichia coli strain G749

Toxin (Protein)


Gene name yoeB Uniprot ID A0A7A2Z7A8
Locus tag AVR74_RS10575 Protein ID WP_024705227.1
Coordinates 2090399..2090653 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AVR74_RS10580 Protein ID WP_001259255.1
Coordinates 2090650..2090901 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AVR74_RS10560 2086892..2088250 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
AVR74_RS28645 2088240..2088302 - 63 WP_010723108.1 membrane protein YoeI -
AVR74_RS10565 2088517..2089446 - 930 WP_000803366.1 LysR family transcriptional regulator -
AVR74_RS10570 2089492..2090316 - 825 WP_000754737.1 SDR family oxidoreductase -
AVR74_RS10575 2090399..2090653 - 255 WP_024705227.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AVR74_RS10580 2090650..2090901 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AVR74_RS26795 2091184..2091234 + 51 WP_001364200.1 his operon leader peptide -
AVR74_RS10585 2091380..2092279 + 900 WP_000131756.1 ATP phosphoribosyltransferase -
AVR74_RS10590 2092285..2093589 + 1305 WP_061157915.1 histidinol dehydrogenase -
AVR74_RS10595 2093586..2094656 + 1071 WP_061157916.1 histidinol-phosphate transaminase -
AVR74_RS10600 2094656..2095723 + 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T61282 WP_024705227.1 NZ_CP014488:c2090653-2090399 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T61282 NZ_CP014488:c2090653-2090399 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT61282 WP_001259255.1 NZ_CP014488:c2090901-2090650 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT61282 NZ_CP014488:c2090901-2090650 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACCATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7A2Z7A8

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References