Detailed information of TA system TA061258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 20355..20940
• Replicon: plasmid pOXA58-AP_882
• Accession: NZ_CP014479
• Organism: Acinetobacter pittii strain AP_882

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8R027
• Locus tag: AYJ52_RS19635
• Protein ID: WP_004698338.1
• Coordinates: 20620..20940 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AYJ52_RS19630
• Protein ID: WP_000369781.1
• Coordinates: 20355..20627 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYJ52_RS19610	16395..17564	+	1170	WP_004698297.1	MobA/MobL family protein	-
AYJ52_RS19615	18112..18993	+	882	WP_005804946.1	replication initiation protein RepM	-
AYJ52_RS19620	19057..19623	+	567	WP_000858429.1	plasmid replication DNA-binding protein	-
AYJ52_RS19625	19974..20291	+	318	WP_005065053.1	hypothetical protein	-
AYJ52_RS19630	20355..20627	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
AYJ52_RS19635	20620..20940	-	321	WP_004698338.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYJ52_RS19640	21107..21367	+	261	WP_004698336.1	hypothetical protein	-
AYJ52_RS19645	21643..21867	-	225	WP_004676219.1	helix-turn-helix domain-containing protein	-
AYJ52_RS19650	22018..22263	+	246	WP_004282220.1	hypothetical protein	-
AYJ52_RS19655	22342..23046	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
AYJ52_RS19660	23109..23501	+	393	Protein_23	recombinase family protein	-
AYJ52_RS19665	23722..24795	+	1074	WP_043041639.1	hypothetical protein	-
AYJ52_RS19670	25239..25535	+	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	-
AYJ52_RS19675	25537..25827	+	291	WP_004969304.1	putative addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / blaOXA-58	-	1..36862	36862
	-	inside	IScluster/Tn	blaOXA-58	-	22342..31046	8704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12222.01 Da
• Isoelectric Point: 6.9800

>T61258 WP_004698338.1 NZ_CP014479:c20940-20620 [Acinetobacter pittii]
MLFIETSIFTKQIKDLVSDEEYRQLQEDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLLAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T61258 NZ_CP014479:c20940-20620 [Acinetobacter pittii]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGGA
AGATCTTTTGGTACAGCCTGATAGAGGTGATCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGAAAAAGTGGTGGTATTCGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATATTTTTCTTATTGGCTTAT
CCAAAATCAGTGAAAGATAATTTAACAGATAAGGAAACAGCCATTCTGCGCCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT61258 WP_000369781.1 NZ_CP014479:c20627-20355 [Acinetobacter pittii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT61258 NZ_CP014479:c20627-20355 [Acinetobacter pittii]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
TCGAGTTACAGAACTCGAACTACCAGATATTAAAGAAGTACGTGAAAAAACAGGCTTGAGCCAAAATGAGTTTGCTGCAC
GTCTGCATATTAGTCCTCGTACCCTGCAAAATTGGGAACAAGGTCGACGCTATCCAACTGGACCAGCAGCTACATTAATT
CGAATTTTAGATGCTCATCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: N8R027