Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 556853..557468 Replicon chromosome
Accession NZ_CP014476
Organism Methylomonas denitrificans strain FJG1

Toxin (Protein)


Gene name higB Uniprot ID A0A140E4P0
Locus tag JT25_RS02475 Protein ID WP_036278447.1
Coordinates 557142..557468 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A140E4N9
Locus tag JT25_RS02470 Protein ID WP_036278442.1
Coordinates 556853..557152 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JT25_RS23645 552718..552939 + 222 WP_082769106.1 hypothetical protein -
JT25_RS02450 553058..554362 + 1305 WP_036272015.1 DUF3422 domain-containing protein -
JT25_RS02455 554541..554765 - 225 WP_036272014.1 hypothetical protein -
JT25_RS02465 555147..556273 + 1127 WP_148655014.1 IS3 family transposase -
JT25_RS23650 556372..556518 + 147 WP_082769351.1 hypothetical protein -
JT25_RS02470 556853..557152 - 300 WP_036278442.1 helix-turn-helix domain-containing protein Antitoxin
JT25_RS02475 557142..557468 - 327 WP_036278447.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JT25_RS23655 557682..557978 - 297 WP_082769107.1 IS3 family transposase -
JT25_RS02480 557924..558529 - 606 WP_132325456.1 IS3 family transposase -
JT25_RS23660 558505..558921 - 417 WP_082769109.1 transposase -
JT25_RS02490 558945..559739 - 795 WP_036278441.1 ethanolamine ammonia-lyase subunit EutC -
JT25_RS02495 559742..561124 - 1383 WP_062327477.1 ethanolamine ammonia-lyase subunit EutB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 555449..588263 32814
- flank IS/Tn - - 555147..555452 305


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-99)

Antitoxin

Cro/C1-type helix-turn-helix domain

(37-85)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12348.28 Da        Isoelectric Point: 10.0240

>T61248 WP_036278447.1 NZ_CP014476:c557468-557142 [Methylomonas denitrificans]
MVIIETSIFTKKIAAILNDDEYRELQTVLVKMPDAGEIIQGSGGIRKIRWGASGRGKRGGARIIYYWATSHNQIFMLFAY
AKNERDNLTKEQLSLLREVVVAEFKNER

Download         Length: 327 bp

>T61248 NZ_CP014476:c557468-557142 [Methylomonas denitrificans]
ATGGTCATTATTGAAACCTCGATCTTTACAAAGAAAATTGCCGCCATCTTGAATGATGATGAATATCGAGAGCTACAAAC
GGTGTTGGTAAAAATGCCTGATGCCGGGGAAATTATTCAGGGCAGTGGTGGAATACGTAAAATTCGTTGGGGCGCCAGTG
GGCGAGGTAAAAGAGGTGGTGCAAGGATCATCTATTATTGGGCTACAAGTCACAATCAGATTTTCATGCTTTTTGCGTAC
GCAAAAAATGAACGTGATAATTTAACCAAAGAACAATTGTCCCTGCTGAGGGAAGTTGTTGTTGCGGAGTTTAAAAATGA
ACGATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10874.43 Da        Isoelectric Point: 8.7787

>AT61248 WP_036278442.1 NZ_CP014476:c557152-556853 [Methylomonas denitrificans]
MNDESFDELIASVKDAGKILRGEVAASRVFDIPEPDVKAIREGIGFSQSKFAHLIGVNLRTLQNWEQGHRHPTGPAKVLL
KLVQADPKSVVKNLHANHA

Download         Length: 300 bp

>AT61248 NZ_CP014476:c557152-556853 [Methylomonas denitrificans]
ATGAACGATGAATCTTTTGACGAACTAATTGCCAGCGTTAAAGATGCTGGGAAAATCCTGCGTGGTGAAGTCGCTGCTTC
ACGTGTGTTCGACATTCCAGAGCCGGACGTTAAAGCGATTCGAGAAGGCATAGGATTTTCTCAATCAAAATTTGCTCACT
TGATTGGAGTGAACCTTAGGACGCTGCAAAACTGGGAGCAAGGCCATCGCCATCCTACAGGGCCTGCCAAAGTGTTGTTA
AAGTTGGTACAAGCCGATCCAAAGTCGGTTGTCAAAAACCTGCATGCAAATCATGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140E4P0

Antitoxin

Source ID Structure
AlphaFold DB A0A140E4N9

References