Detailed information of TA system TA060825

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF3/-
• Location: 2122727..2123026
• Replicon: chromosome
• Accession: NZ_CP014365
• Organism: Staphylococcus aureus strain USA300-SUR2

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: AYM14_RS11175
• Protein ID: WP_011447039.1
• Coordinates: 2122850..2123026 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	2122727..2122782 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYM14_RS11130	2118058..2118318	+	261	WP_001791826.1	hypothetical protein	-
AYM14_RS11135	2118371..2118721	-	351	WP_000702263.1	complement inhibitor SCIN-A	-
AYM14_RS11140	2119406..2119855	+	450	WP_000727649.1	chemotaxis-inhibiting protein CHIPS	-
AYM14_RS11145	2119950..2120285	-	336	Protein_2060	SH3 domain-containing protein	-
AYM14_RS11155	2120935..2121426	-	492	WP_000919350.1	staphylokinase	-
AYM14_RS11160	2121617..2122372	-	756	WP_000861038.1	CHAP domain-containing protein	-
AYM14_RS11165	2122384..2122638	-	255	WP_000611512.1	phage holin	-
AYM14_RS11170	2122690..2122797	+	108	WP_001791821.1	hypothetical protein	-
-	2122719..2122858	+	140	NuclAT_0	-	-
-	2122719..2122858	+	140	NuclAT_0	-	-
-	2122719..2122858	+	140	NuclAT_0	-	-
-	2122719..2122858	+	140	NuclAT_0	-	-
-	2122727..2122782	+	56	-	-	Antitoxin
AYM14_RS11175	2122850..2123026	-	177	WP_011447039.1	putative holin-like toxin	Toxin
AYM14_RS11180	2123176..2123472	-	297	WP_000539688.1	DUF2951 domain-containing protein	-
AYM14_RS11185	2123530..2123817	-	288	WP_001040261.1	hypothetical protein	-
AYM14_RS11190	2123864..2124016	-	153	WP_001153681.1	hypothetical protein	-
AYM14_RS11195	2124006..2127791	-	3786	WP_000582165.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / chp / sak / hlb / groEL	2118371..2174318	55947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T60825 WP_011447039.1 NZ_CP014365:c2123026-2122850 [Staphylococcus aureus]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T60825 NZ_CP014365:c2123026-2122850 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 56 bp

>AT60825 NZ_CP014365:2122727-2122782 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Structures

Toxin

• PDB: 7NS1

Antitoxin