Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3245175..3245677 Replicon chromosome
Accession NZ_CP014348
Organism Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AWN69_RS15870 Protein ID WP_000767829.1
Coordinates 3245423..3245677 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AWN69_RS15865 Protein ID WP_001259255.1
Coordinates 3245175..3245426 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWN69_RS15845 3240353..3241420 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AWN69_RS15850 3241420..3242490 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
AWN69_RS15855 3242487..3243791 - 1305 WP_000009594.1 histidinol dehydrogenase -
AWN69_RS15860 3243797..3244696 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AWN69_RS24405 3244842..3244892 - 51 WP_001364200.1 his operon leader peptide -
AWN69_RS15865 3245175..3245426 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AWN69_RS15870 3245423..3245677 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AWN69_RS15875 3245760..3246584 + 825 WP_000754737.1 SDR family oxidoreductase -
AWN69_RS15880 3246630..3247559 + 930 WP_000803351.1 LysR family transcriptional regulator -
AWN69_RS25835 3247774..3247836 + 63 WP_010723108.1 membrane protein YoeI -
AWN69_RS15885 3247826..3249184 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AWN69_RS15890 3249363..3250421 + 1059 WP_000492339.1 transport protein YeeE -
AWN69_RS15895 3250435..3250662 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T60769 WP_000767829.1 NZ_CP014348:3245423-3245677 [Escherichia coli str. K-12 substr. MG1655]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T60769 NZ_CP014348:3245423-3245677 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT60769 WP_001259255.1 NZ_CP014348:3245175-3245426 [Escherichia coli str. K-12 substr. MG1655]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT60769 NZ_CP014348:3245175-3245426 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References