60769

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3245175..3245677 Replicon chromosome
Accession NZ_CP014348
Organism Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AWN69_RS15870 Protein ID WP_000767829.1
Coordinates 3245423..3245677 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AWN69_RS15865 Protein ID WP_001259255.1
Coordinates 3245175..3245426 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AWN69_RS15845 3240353..3241420 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AWN69_RS15850 3241420..3242490 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
AWN69_RS15855 3242487..3243791 - 1305 WP_000009594.1 histidinol dehydrogenase -
AWN69_RS15860 3243797..3244696 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AWN69_RS24405 3244842..3244892 - 51 WP_001364200.1 his operon leader peptide -
AWN69_RS15865 3245175..3245426 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AWN69_RS15870 3245423..3245677 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AWN69_RS15875 3245760..3246584 + 825 WP_000754737.1 SDR family oxidoreductase -
AWN69_RS15880 3246630..3247559 + 930 WP_000803351.1 LysR family transcriptional regulator -
AWN69_RS25835 3247774..3247836 + 63 WP_010723108.1 membrane protein YoeI -
AWN69_RS15885 3247826..3249184 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AWN69_RS15890 3249363..3250421 + 1059 WP_000492339.1 transport protein YeeE -
AWN69_RS15895 3250435..3250662 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T60769 WP_000767829.1 NZ_CP014348:3245423-3245677 [Escherichia coli str. K-12 substr. MG1655]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T60769 NZ_CP014348:3245423-3245677 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT60769 WP_001259255.1 NZ_CP014348:3245175-3245426 [Escherichia coli str. K-12 substr. MG1655]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT60769 NZ_CP014348:3245175-3245426 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References