Detailed information of TA system TA060754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 452126..452667
• Replicon: chromosome
• Accession: NZ_CP014348
• Organism: Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q47149
• Locus tag: AWN69_RS02320
• Protein ID: WP_000615983.1
• Coordinates: 452389..452667 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: Q47150
• Locus tag: AWN69_RS02315
• Protein ID: WP_000729703.1
• Coordinates: 452126..452386 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AWN69_RS02295	447801..448586	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
AWN69_RS02300	448558..450270	+	1713	Protein_452	flagellar biosynthesis protein FlhA	-
AWN69_RS02305	450494..450991	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
AWN69_RS02310	451167..451916	-	750	WP_000056849.1	C40 family peptidase	-
AWN69_RS02315	452126..452386	+	261	WP_000729703.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
AWN69_RS02320	452389..452667	+	279	WP_000615983.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
AWN69_RS02325	452823..453563	+	741	WP_001225679.1	murein L,D-transpeptidase	-
AWN69_RS02330	453534..454301	-	768	WP_000333380.1	class II glutamine amidotransferase	-
AWN69_RS02335	454507..455085	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10846.62 Da
• Isoelectric Point: 9.9941

>T60754 WP_000615983.1 NZ_CP014348:452389-452667 [Escherichia coli str. K-12 substr. MG1655]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T60754 NZ_CP014348:452389-452667 [Escherichia coli str. K-12 substr. MG1655]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9405.80 Da
• Isoelectric Point: 5.0758

>AT60754 WP_000729703.1 NZ_CP014348:452126-452386 [Escherichia coli str. K-12 substr. MG1655]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT60754 NZ_CP014348:452126-452386 [Escherichia coli str. K-12 substr. MG1655]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 4Q2U

Antitoxin

• AlphaFold DB: A0A5F1F716