Detailed information of TA system TA060753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 445929..446623
• Replicon: chromosome
• Accession: NZ_CP014348
• Organism: Escherichia coli str. K-12 substr. MG1655 strain JW5437-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: AWN69_RS02280
• Protein ID: WP_001263489.1
• Coordinates: 445929..446327 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AWN69_RS02285
• Protein ID: WP_000554758.1
• Coordinates: 446330..446623 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	441517..441597	-	81	NuclAT_12	-	-
-	441517..441597	-	81	NuclAT_12	-	-
-	441517..441597	-	81	NuclAT_12	-	-
-	441517..441597	-	81	NuclAT_12	-	-
AWN69_RS02255	442193..442651	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AWN69_RS02260	442912..444369	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
AWN69_RS02265	444426..444947	-	522	Protein_445	peptide chain release factor H	-
AWN69_RS02270	444943..445149	-	207	Protein_446	RtcB family protein	-
AWN69_RS02275	445467..445919	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
AWN69_RS02280	445929..446327	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AWN69_RS02285	446330..446623	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AWN69_RS02290	446675..447730	-	1056	WP_001226164.1	DNA polymerase IV	-
AWN69_RS02295	447801..448586	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
AWN69_RS02300	448558..450270	+	1713	Protein_452	flagellar biosynthesis protein FlhA	-
AWN69_RS02305	450494..450991	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T60753 WP_001263489.1 NZ_CP014348:c446327-445929 [Escherichia coli str. K-12 substr. MG1655]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T60753 NZ_CP014348:c446327-445929 [Escherichia coli str. K-12 substr. MG1655]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT60753 WP_000554758.1 NZ_CP014348:c446623-446330 [Escherichia coli str. K-12 substr. MG1655]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT60753 NZ_CP014348:c446623-446330 [Escherichia coli str. K-12 substr. MG1655]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3