Detailed information of TA system TA060735

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 93331..93600
• Replicon: plasmid pJJ1887-4
• Accession: NZ_CP014321
• Organism: Escherichia coli JJ1887

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AX202_RS26885
• Protein ID: WP_001312861.1
• Coordinates: 93442..93600 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	93331..93396 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX202_RS26855	89124..89651	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
AX202_RS26860	89707..89940	+	234	WP_000006004.1	DUF905 domain-containing protein	-
AX202_RS26865	89999..91957	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
AX202_RS26870	92012..92446	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
AX202_RS26875	92443..93162	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
AX202_RS30390	93174..93362	-	189	WP_001299721.1	hypothetical protein	-
-	93174..93398	+	225	NuclAT_0	-	-
-	93174..93398	+	225	NuclAT_0	-	-
-	93174..93398	+	225	NuclAT_0	-	-
-	93174..93398	+	225	NuclAT_0	-	-
-	93331..93396	+	66	-	-	Antitoxin
AX202_RS26885	93442..93600	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
AX202_RS26890	93951..94655	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
AX202_RS26895	94708..96603	+	1896	Protein_116	Tn3 family transposase	-
AX202_RS26900	96702..97355	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
AX202_RS30330	97448..97705	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
AX202_RS26910	97638..98039	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B	-	1..107507	107507

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T60735 WP_001312861.1 NZ_CP014321:93442-93600 [Escherichia coli JJ1887]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T60735 NZ_CP014321:93442-93600 [Escherichia coli JJ1887]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT60735 NZ_CP014321:93331-93396 [Escherichia coli JJ1887]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin