Detailed information of TA system TA060723

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 847..1101
• Replicon: plasmid pJJ1887-5
• Accession: NZ_CP014320
• Organism: Escherichia coli JJ1887

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: AX202_RS26965
• Protein ID: WP_032215358.1
• Coordinates: 847..1053 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	1040..1101 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX202_RS25560	306..563	-	258	WP_000083833.1	replication regulatory protein RepA	-
AX202_RS26965	847..1053	-	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	1040..1101	+	62	NuclAT_0	-	Antitoxin
-	1040..1101	+	62	NuclAT_0	-	Antitoxin
-	1040..1101	+	62	NuclAT_0	-	Antitoxin
-	1040..1101	+	62	NuclAT_0	-	Antitoxin
AX202_RS30625	1240..1506	-	267	WP_087757657.1	hypothetical protein	-
AX202_RS25575	1881..2450	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
AX202_RS25580	2602..3228	-	627	WP_029702149.1	hypothetical protein	-
AX202_RS25585	3640..3849	-	210	WP_039023209.1	hypothetical protein	-
AX202_RS25590	3980..4540	-	561	WP_001567328.1	fertility inhibition protein FinO	-
AX202_RS25595	4595..5341	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / blaTEM-1B / aac(3)-IIa / mph(A) / sul1 / qacE / dfrB4 / qepA1	-	1..130603	130603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T60723 WP_032215358.1 NZ_CP014320:c1053-847 [Escherichia coli JJ1887]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T60723 NZ_CP014320:c1053-847 [Escherichia coli JJ1887]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT60723 NZ_CP014320:1040-1101 [Escherichia coli JJ1887]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin