Detailed information of TA system TA060717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4430076..4430678
• Replicon: chromosome
• Accession: NZ_CP014316
• Organism: Escherichia coli JJ1887

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AX202_RS22280
• Protein ID: WP_000897302.1
• Coordinates: 4430076..4430387 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AX202_RS22285
• Protein ID: WP_000356397.1
• Coordinates: 4430388..4430678 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX202_RS22255	4425990..4426589	+	600	WP_001296610.1	glucose-1-phosphatase	-
AX202_RS22260	4426583..4427455	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AX202_RS22265	4427452..4427889	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AX202_RS22270	4427934..4428875	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AX202_RS22275	4428939..4429847	-	909	WP_001385591.1	alpha/beta hydrolase	-
AX202_RS22280	4430076..4430387	+	312	WP_000897302.1	hypothetical protein	Toxin
AX202_RS22285	4430388..4430678	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AX202_RS22295	4431037..4431315	+	279	WP_001296612.1	hypothetical protein	-
AX202_RS22300	4431712..4431930	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
AX202_RS22305	4432115..4432564	-	450	WP_032140890.1	hypothetical protein	-
AX202_RS22310	4432880..4433728	-	849	WP_001038650.1	hypothetical protein	-
AX202_RS22315	4434018..4434260	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
AX202_RS22320	4434442..4435371	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T60717 WP_000897302.1 NZ_CP014316:4430076-4430387 [Escherichia coli JJ1887]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T60717 NZ_CP014316:4430076-4430387 [Escherichia coli JJ1887]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT60717 WP_000356397.1 NZ_CP014316:4430388-4430678 [Escherichia coli JJ1887]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT60717 NZ_CP014316:4430388-4430678 [Escherichia coli JJ1887]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416