Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3620851..3621650 Replicon chromosome
Accession NZ_CP014316
Organism Escherichia coli JJ1887

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag AX202_RS18265 Protein ID WP_000347251.1
Coordinates 3621186..3621650 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag AX202_RS18260 Protein ID WP_001296435.1
Coordinates 3620851..3621186 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AX202_RS18245 3616636..3617406 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
AX202_RS18250 3617422..3618756 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
AX202_RS18255 3619131..3620702 + 1572 WP_001273763.1 galactarate dehydratase -
AX202_RS18260 3620851..3621186 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AX202_RS18265 3621186..3621650 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AX202_RS18270 3621705..3622514 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
AX202_RS18275 3622763..3624043 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
AX202_RS18280 3624066..3624539 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
AX202_RS18285 3624550..3625329 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
AX202_RS18290 3625319..3626197 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
AX202_RS18295 3626215..3626649 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T60715 WP_000347251.1 NZ_CP014316:3621186-3621650 [Escherichia coli JJ1887]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T60715 NZ_CP014316:3621186-3621650 [Escherichia coli JJ1887]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT60715 WP_001296435.1 NZ_CP014316:3620851-3621186 [Escherichia coli JJ1887]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT60715 NZ_CP014316:3620851-3621186 [Escherichia coli JJ1887]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References