Detailed information of TA system TA060689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 5372714..5373513
• Replicon: chromosome
• Accession: NZ_CP014314
• Organism: Escherichia coli O157:H7 strain JEONG-1266

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: JEONG1266_RS27590
• Protein ID: WP_000347273.1
• Coordinates: 5372714..5373178 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: JEONG1266_RS27595
• Protein ID: WP_001302819.1
• Coordinates: 5373178..5373513 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEONG1266_RS27560	5367715..5368149	-	435	WP_000948831.1	PTS sugar transporter subunit IIA	-
JEONG1266_RS27565	5368167..5369045	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JEONG1266_RS27570	5369035..5369814	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JEONG1266_RS27575	5369825..5370298	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JEONG1266_RS27580	5370321..5371601	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JEONG1266_RS27585	5371850..5372659	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
JEONG1266_RS27590	5372714..5373178	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JEONG1266_RS27595	5373178..5373513	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JEONG1266_RS27600	5373662..5375233	-	1572	WP_001273776.1	galactarate dehydratase	-
JEONG1266_RS27605	5375608..5376942	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
JEONG1266_RS27610	5376958..5377728	+	771	WP_001058231.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T60689 WP_000347273.1 NZ_CP014314:c5373178-5372714 [Escherichia coli O157:H7]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T60689 NZ_CP014314:c5373178-5372714 [Escherichia coli O157:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT60689 WP_001302819.1 NZ_CP014314:c5373513-5373178 [Escherichia coli O157:H7]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT60689 NZ_CP014314:c5373513-5373178 [Escherichia coli O157:H7]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7