Detailed information of TA system TA060679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3627504..3628198
• Replicon: chromosome
• Accession: NZ_CP014314
• Organism: Escherichia coli O157:H7 strain JEONG-1266

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: JEONG1266_RS19030
• Protein ID: WP_001263495.1
• Coordinates: 3627504..3627902 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JEONG1266_RS19035
• Protein ID: WP_000554757.1
• Coordinates: 3627905..3628198 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3623091..3623171	-	81	NuclAT_11	-	-
-	3623091..3623171	-	81	NuclAT_11	-	-
-	3623091..3623171	-	81	NuclAT_11	-	-
-	3623091..3623171	-	81	NuclAT_11	-	-
JEONG1266_RS19005	3623767..3624225	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JEONG1266_RS19010	3624486..3625943	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JEONG1266_RS19015	3626000..3626521	-	522	Protein_3616	peptide chain release factor H	-
JEONG1266_RS19020	3626520..3626723	-	204	Protein_3617	RNA ligase RtcB family protein	-
JEONG1266_RS19025	3627042..3627494	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
JEONG1266_RS19030	3627504..3627902	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JEONG1266_RS19035	3627905..3628198	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JEONG1266_RS19040	3628250..3629305	-	1056	WP_001226186.1	DNA polymerase IV	-
JEONG1266_RS19045	3629376..3630161	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
JEONG1266_RS19050	3630133..3631845	+	1713	Protein_3623	flagellar biosynthesis protein FlhA	-
JEONG1266_RS32725	3631990..3632487	-	498	Protein_3624	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T60679 WP_001263495.1 NZ_CP014314:c3627902-3627504 [Escherichia coli O157:H7]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T60679 NZ_CP014314:c3627902-3627504 [Escherichia coli O157:H7]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT60679 WP_000554757.1 NZ_CP014314:c3628198-3627905 [Escherichia coli O157:H7]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT60679 NZ_CP014314:c3628198-3627905 [Escherichia coli O157:H7]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6