Detailed information of TA system TA060674

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2742236..2742461
• Replicon: chromosome
• Accession: NZ_CP014314
• Organism: Escherichia coli O157:H7 strain JEONG-1266

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: JEONG1266_RS14770
• Protein ID: WP_000813263.1
• Coordinates: 2742236..2742391 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2742403..2742461 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEONG1266_RS14735	2737690..2738403	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
JEONG1266_RS14740	2738541..2738737	-	197	Protein_2807	TrmB family transcriptional regulator	-
JEONG1266_RS14745	2739024..2739842	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
JEONG1266_RS14750	2739994..2740365	-	372	WP_000090264.1	antitermination protein	-
JEONG1266_RS14755	2740355..2740726	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
JEONG1266_RS14760	2740739..2741788	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
JEONG1266_RS31630	2741790..2742068	-	279	WP_001341388.1	hypothetical protein	-
JEONG1266_RS14770	2742236..2742391	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	2742403..2742461	+	59	-	-	Antitoxin
JEONG1266_RS14775	2742996..2743769	+	774	WP_000160654.1	alpha/beta hydrolase	-
JEONG1266_RS14780	2744121..2744534	-	414	WP_001151233.1	DUF977 family protein	-
JEONG1266_RS14785	2744550..2745320	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
JEONG1266_RS14790	2745342..2746088	-	747	WP_000788745.1	ATP-binding protein	-
JEONG1266_RS14795	2746095..2747186	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T60674 WP_000813263.1 NZ_CP014314:c2742391-2742236 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T60674 NZ_CP014314:c2742391-2742236 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT60674 NZ_CP014314:2742403-2742461 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin