Detailed information of TA system TA060670

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1865663..1865888
• Replicon: chromosome
• Accession: NZ_CP014314
• Organism: Escherichia coli O157:H7 strain JEONG-1266

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: JEONG1266_RS10005
• Protein ID: WP_000813258.1
• Coordinates: 1865733..1865888 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1865663..1865721 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JEONG1266_RS09955	1860728..1860970	+	243	WP_000747948.1	hypothetical protein	-
JEONG1266_RS09960	1860954..1861379	+	426	WP_000693878.1	Rha family transcriptional regulator	-
JEONG1266_RS32365	1861448..1862491	+	1044	WP_001262402.1	hypothetical protein	-
JEONG1266_RS32370	1862484..1862945	+	462	WP_000139447.1	replication protein	-
JEONG1266_RS09975	1862979..1863695	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
JEONG1266_RS09980	1863728..1864009	+	282	WP_000603384.1	DNA-binding protein	-
JEONG1266_RS09985	1864006..1864233	+	228	WP_000699809.1	hypothetical protein	-
JEONG1266_RS09990	1864226..1864537	+	312	WP_001289673.1	hypothetical protein	-
JEONG1266_RS09995	1864665..1864883	+	219	WP_000683609.1	DUF4014 family protein	-
JEONG1266_RS10000	1864885..1865442	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	1865663..1865721	-	59	-	-	Antitoxin
JEONG1266_RS10005	1865733..1865888	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
JEONG1266_RS10010	1866008..1866352	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
JEONG1266_RS10015	1866474..1866746	+	273	WP_000191872.1	hypothetical protein	-
JEONG1266_RS10020	1866748..1867797	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
JEONG1266_RS10025	1867810..1868115	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
JEONG1266_RS10030	1868178..1868732	+	555	WP_000640035.1	DUF1133 family protein	-
JEONG1266_RS32375	1868957..1869154	+	198	WP_000917763.1	hypothetical protein	-
JEONG1266_RS10040	1869290..1870003	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
JEONG1266_RS10055	1870454..1870885	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	1797272..1902010	104738

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T60670 WP_000813258.1 NZ_CP014314:1865733-1865888 [Escherichia coli O157:H7]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T60670 NZ_CP014314:1865733-1865888 [Escherichia coli O157:H7]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT60670 NZ_CP014314:c1865721-1865663 [Escherichia coli O157:H7]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin