Detailed information of TA system TA060646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 620870..621570
• Replicon: chromosome
• Accession: NZ_CP014307
• Organism: Burkholderia sp. PAMC 26561

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: A0A109WU43
• Locus tag: AXG89_RS18455
• Protein ID: WP_062171488.1
• Coordinates: 621274..621570 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: -
• Locus tag: AXG89_RS18450
• Protein ID: WP_062003305.1
• Coordinates: 620870..621271 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXG89_RS18430	617399..617845	-	447	WP_082771542.1	GFA family protein	-
AXG89_RS18435	618009..619328	+	1320	WP_062171484.1	hypothetical protein	-
AXG89_RS18440	619363..619854	+	492	WP_062172584.1	isochorismatase family protein	-
AXG89_RS18445	619998..620795	+	798	WP_062171486.1	transporter substrate-binding domain-containing protein	-
AXG89_RS18450	620870..621271	-	402	WP_062003305.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
AXG89_RS18455	621274..621570	-	297	WP_062171488.1	type II toxin-antitoxin system MqsR family toxin	Toxin
AXG89_RS18460	621876..622574	+	699	WP_062003306.1	2Fe-2S iron-sulfur cluster binding domain-containing protein	-
AXG89_RS18465	622584..623573	+	990	WP_062171490.1	xanthine dehydrogenase family protein subunit M	-
AXG89_RS18470	623570..625798	+	2229	WP_062171492.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
AXG89_RS18475	625924..626511	+	588	WP_062171493.1	nucleotidyltransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-127)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10831.66 Da
• Isoelectric Point: 6.2078

>T60646 WP_062171488.1 NZ_CP014307:c621570-621274 [Burkholderia sp. PAMC 26561]
MEKSVPHCRLAIVLALLEAGMVRSTMSALSGAAAMGFDFNDMIEVIRALAPKDFYKSMTTHADHRVWQDVYQPTTSAGPV
YLKLTVIDDVLVVSFKEL

Nucleotide

• Length: 297 bp

>T60646 NZ_CP014307:c621570-621274 [Burkholderia sp. PAMC 26561]
ATGGAAAAAAGCGTCCCTCACTGCCGGCTTGCGATCGTTCTCGCTCTGCTCGAAGCGGGCATGGTGCGCTCGACCATGTC
AGCACTGAGTGGCGCGGCGGCAATGGGTTTTGACTTCAACGACATGATCGAAGTCATCAGGGCATTGGCGCCAAAAGATT
TTTATAAGAGCATGACAACGCACGCCGATCACCGCGTTTGGCAGGACGTCTACCAACCAACCACATCCGCCGGGCCGGTG
TACTTGAAACTGACGGTCATAGATGACGTGCTTGTGGTGTCCTTCAAGGAGCTATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14522.74 Da
• Isoelectric Point: 5.2321

>AT60646 WP_062003305.1 NZ_CP014307:c621271-620870 [Burkholderia sp. PAMC 26561]
MKCPVCGAAELVRDTRDLPYAYKGESTMIPDVTGDFCPACGEAIFDATESLRINAAMLEFNKQVNATIVDPAFIASVRKK
LALDQREAAEIFGGGVNAFSRYENGKTRPPLALVKLLKILDHHPDLLEEVRAA

Nucleotide

• Length: 402 bp

>AT60646 NZ_CP014307:c621271-620870 [Burkholderia sp. PAMC 26561]
ATGAAGTGTCCAGTCTGCGGCGCAGCAGAACTCGTTCGTGATACACGCGACTTGCCATACGCGTACAAAGGCGAGTCGAC
AATGATCCCTGACGTAACCGGCGACTTTTGCCCGGCGTGTGGCGAAGCCATTTTCGATGCGACCGAGTCGTTGCGCATCA
ATGCAGCGATGCTGGAATTCAACAAGCAAGTCAACGCAACGATTGTCGATCCGGCTTTTATTGCAAGCGTGCGCAAAAAG
CTTGCTCTCGATCAACGCGAAGCGGCCGAAATATTTGGCGGTGGTGTGAACGCGTTTTCCCGCTACGAAAACGGTAAGAC
AAGGCCGCCTTTGGCCTTGGTGAAGCTGTTGAAGATTCTGGATCACCACCCTGATCTGTTGGAAGAGGTCAGAGCTGCAT
GA

Structures

Toxin

• AlphaFold DB: A0A109WU43