Detailed information of TA system TA060644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2508444..2509040
• Replicon: chromosome
• Accession: NZ_CP014306
• Organism: Burkholderia sp. PAMC 26561

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A242MGE3
• Locus tag: AXG89_RS11535
• Protein ID: WP_062000798.1
• Coordinates: 2508762..2509040 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AXG89_RS11530
• Protein ID: WP_061999063.1
• Coordinates: 2508444..2508746 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AXG89_RS11520	2505559..2507265	-	1707	WP_062169755.1	single-stranded-DNA-specific exonuclease RecJ	-
AXG89_RS11525	2507278..2508399	-	1122	WP_062169756.1	hypothetical protein	-
AXG89_RS11530	2508444..2508746	-	303	WP_061999063.1	HigA family addiction module antidote protein	Antitoxin
AXG89_RS11535	2508762..2509040	-	279	WP_062000798.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AXG89_RS11540	2509439..2510692	+	1254	WP_061999064.1	lipoprotein-releasing ABC transporter permease subunit	-
AXG89_RS11545	2510685..2511392	+	708	WP_061999065.1	lipoprotein-releasing ABC transporter ATP-binding protein LolD	-
AXG89_RS11550	2511415..2512203	+	789	WP_061999066.1	TatD family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-81)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10602.08 Da
• Isoelectric Point: 9.7808

>T60644 WP_062000798.1 NZ_CP014306:c2509040-2508762 [Burkholderia sp. PAMC 26561]
MIQTFRCKDTQALYEGETPKRFAAIADVATRRLSALDASHELRDLKSPPGNRLERLSGDRRGQLSIRINSQWRVCFKWTT
KGPVDVEIIDYH

Nucleotide

• Length: 279 bp

>T60644 NZ_CP014306:c2509040-2508762 [Burkholderia sp. PAMC 26561]
ATGATTCAAACGTTCCGATGCAAGGACACCCAGGCACTTTACGAAGGTGAAACGCCGAAGCGGTTCGCAGCAATTGCAGA
TGTCGCGACCAGAAGGCTTTCGGCGCTGGATGCTTCGCACGAGTTGCGCGACCTGAAATCACCGCCCGGCAACCGTCTCG
AACGGCTGTCGGGCGACCGGCGGGGCCAGCTCAGCATTCGGATCAACAGCCAGTGGCGCGTTTGTTTCAAATGGACGACG
AAGGGTCCTGTCGACGTCGAGATCATTGACTATCACTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11522.27 Da
• Isoelectric Point: 8.4844

>AT60644 WP_061999063.1 NZ_CP014306:c2508746-2508444 [Burkholderia sp. PAMC 26561]
MSKNGMRAVHPGEILREDYLVPLELSVNALALALNVPATRLHEIVKERRGITADTAYRLARYFDTTPEFWLNLQAMYDLK
TLPTRSEIDRKVEPREPTRA

Nucleotide

• Length: 303 bp

>AT60644 NZ_CP014306:c2508746-2508444 [Burkholderia sp. PAMC 26561]
ATGTCGAAGAACGGAATGCGCGCAGTTCATCCCGGAGAAATTCTCCGGGAAGACTATCTGGTTCCGCTGGAGTTGAGCGT
GAACGCGCTGGCGCTGGCGCTCAACGTGCCGGCCACGCGGTTGCACGAGATCGTGAAGGAACGGCGCGGCATCACGGCGG
ATACGGCTTATCGCCTCGCGCGCTACTTCGACACCACGCCTGAATTCTGGTTGAATCTGCAGGCCATGTACGACCTCAAG
ACACTGCCCACACGCAGTGAAATCGATCGAAAAGTCGAACCGCGCGAGCCTACCCGCGCTTAA

Structures

Toxin

• AlphaFold DB: A0A242MGE3