Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3598424..3599015 Replicon chromosome
Accession NZ_CP014301
Organism Bosea sp. PAMC 26642 strain PAMC26642

Toxin (Protein)


Gene name graT Uniprot ID A0A126NY35
Locus tag AXW83_RS17290 Protein ID WP_066615432.1
Coordinates 3598424..3598708 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A126NYS8
Locus tag AXW83_RS17295 Protein ID WP_066615434.1
Coordinates 3598719..3599015 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXW83_RS17270 (AXW83_17275) 3594368..3595171 + 804 WP_066620674.1 transglutaminase family protein -
AXW83_RS17275 (AXW83_17280) 3595279..3595728 + 450 WP_066615427.1 transcriptional repressor -
AXW83_RS17280 (AXW83_17285) 3595807..3597054 + 1248 WP_066615428.1 aminopeptidase -
AXW83_RS17285 (AXW83_17290) 3597087..3598340 + 1254 WP_066615430.1 MFS transporter -
AXW83_RS17290 (AXW83_17295) 3598424..3598708 + 285 WP_066615432.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AXW83_RS17295 (AXW83_17300) 3598719..3599015 + 297 WP_066615434.1 HigA family addiction module antidote protein Antitoxin
AXW83_RS17300 (AXW83_17305) 3599058..3601010 - 1953 WP_066615436.1 acetate--CoA ligase -
AXW83_RS17305 (AXW83_17310) 3601263..3602390 + 1128 WP_082767517.1 DUF2235 domain-containing protein -
AXW83_RS17310 (AXW83_17315) 3602401..3602808 - 408 WP_066615440.1 EVE domain-containing protein -
AXW83_RS17315 (AXW83_17320) 3602819..3603157 - 339 WP_066615441.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-94)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-95)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10863.59 Da        Isoelectric Point: 10.0055

>T60632 WP_066615432.1 NZ_CP014301:3598424-3598708 [Bosea sp. PAMC 26642]
MTIQSFKDTRLEAILERRVPKGFPADLMSVTRRKLIMLDSALTLADLRLPPANRLEALKSDRAGQHSIRVNDQFRICFRW
SVHGPADIEFVDYH

Download         Length: 285 bp

>T60632 NZ_CP014301:3598424-3598708 [Bosea sp. PAMC 26642]
ATGACGATCCAGAGTTTCAAGGACACGAGACTCGAGGCAATCCTCGAACGCCGGGTTCCAAAAGGATTCCCGGCTGACCT
CATGTCTGTGACGCGGCGAAAGCTCATTATGCTGGACAGCGCTCTGACACTCGCTGACCTCAGGTTGCCGCCGGCCAATC
GTCTCGAAGCCCTCAAATCCGATCGGGCCGGGCAACATTCGATCCGCGTGAACGACCAGTTCCGCATCTGCTTCCGCTGG
AGCGTCCATGGGCCGGCCGACATCGAATTCGTCGATTATCATTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10981.57 Da        Isoelectric Point: 5.8452

>AT60632 WP_066615434.1 NZ_CP014301:3598719-3599015 [Bosea sp. PAMC 26642]
MTKLSPVHPGEILREEFLAPLDLSSGSVARAIGVPRTRIERLAQEQVDLSPDTAIRLARFFGTSAEFWMAIQSRYALERA
TDRLGDNLLQIEPMRQAS

Download         Length: 297 bp

>AT60632 NZ_CP014301:3598719-3599015 [Bosea sp. PAMC 26642]
ATGACGAAGCTCTCCCCCGTCCACCCCGGAGAAATCCTGCGCGAGGAATTCCTCGCACCTCTCGATTTGTCCTCCGGATC
GGTTGCGCGCGCGATCGGGGTCCCTCGCACGCGGATCGAACGGCTCGCGCAGGAGCAGGTCGACTTGAGCCCGGATACGG
CCATTCGTCTGGCGCGGTTCTTCGGAACCAGCGCCGAGTTCTGGATGGCGATCCAGAGTCGCTACGCCCTGGAACGAGCC
ACAGATCGTCTCGGCGACAACCTGTTGCAGATCGAACCGATGAGGCAGGCGAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A126NY35

Antitoxin

Source ID Structure
AlphaFold DB A0A126NYS8

References