60627

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 409061..409689 Replicon chromosome
Accession NZ_CP014301
Organism Bosea sp. PAMC 26642 strain PAMC26642

Toxin (Protein)


Gene name higB Uniprot ID A0A126NQV8
Locus tag AXW83_RS01895 Protein ID WP_066610137.1
Coordinates 409354..409689 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A126P2M2
Locus tag AXW83_RS26885 Protein ID WP_066619648.1
Coordinates 409061..409309 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXW83_RS01865 (AXW83_01865) 404415..405203 + 789 WP_066610125.1 ABC transporter permease -
AXW83_RS01870 (AXW83_01870) 405304..405573 + 270 WP_066610127.1 hypothetical protein -
AXW83_RS01875 (AXW83_01875) 405638..407803 - 2166 WP_066610131.1 methylmalonyl-CoA mutase -
AXW83_RS01880 (AXW83_01880) 407800..408249 - 450 WP_156639692.1 hypothetical protein -
AXW83_RS01885 (AXW83_01885) 408246..409064 - 819 WP_066610135.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
AXW83_RS26885 (AXW83_01890) 409061..409309 - 249 WP_066619648.1 helix-turn-helix transcriptional regulator Antitoxin
AXW83_RS01895 (AXW83_01895) 409354..409689 - 336 WP_066610137.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AXW83_RS01900 (AXW83_01900) 409702..411588 - 1887 WP_066619651.1 methylmalonyl-CoA mutase subunit beta -
AXW83_RS01905 (AXW83_01905) 411796..412182 - 387 WP_066610139.1 hypothetical protein -
AXW83_RS01910 (AXW83_01910) 412327..413883 + 1557 WP_066610141.1 DUF853 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 409061..433108 24047


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(21-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-74)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12684.88 Da        Isoelectric Point: 10.4559

>T60627 WP_066610137.1 NZ_CP014301:c409689-409354 [Bosea sp. PAMC 26642]
MNLKSGWTFEFLNAAVKAEAGALPRDMRAKLYRYSDLIEDEGLEALPYGWVKPLGDKLWELRLTGRDGIARAIYLAATGK
RVVILRVFLKKTEKTPPKELRIARERARDIP

Download         Length: 336 bp

>T60627 NZ_CP014301:c409689-409354 [Bosea sp. PAMC 26642]
ATGAACCTGAAATCAGGCTGGACGTTCGAGTTCCTGAATGCGGCTGTCAAAGCAGAGGCGGGCGCGCTGCCGCGGGATAT
GCGAGCCAAGCTCTATCGTTATTCCGACCTCATCGAGGATGAAGGCCTTGAGGCTTTGCCCTATGGCTGGGTCAAGCCGC
TTGGCGACAAGCTCTGGGAGCTGCGGTTGACGGGCCGCGACGGGATCGCGAGAGCGATCTATCTGGCTGCGACCGGCAAA
CGGGTCGTCATTCTGCGTGTTTTCCTTAAGAAGACCGAGAAGACGCCGCCGAAGGAACTTCGTATCGCCCGCGAGCGAGC
AAGGGACATTCCGTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9259.49 Da        Isoelectric Point: 9.8240

>AT60627 WP_066619648.1 NZ_CP014301:c409309-409061 [Bosea sp. PAMC 26642]
MKDPDFKREYDALEEEFALLTSLLRARTRAGLTQEELATRMGTTQSVVARLESGRGKPSTRTLERYARATGTRLRITLEA
AE

Download         Length: 249 bp

>AT60627 NZ_CP014301:c409309-409061 [Bosea sp. PAMC 26642]
ATGAAAGACCCCGACTTCAAGCGCGAATACGACGCGTTGGAAGAAGAATTCGCCCTGCTCACCTCCCTGCTGCGCGCCCG
CACCCGTGCCGGGCTGACGCAGGAGGAACTCGCCACTCGCATGGGCACGACGCAATCGGTCGTCGCTCGGCTCGAAAGCG
GCCGGGGCAAGCCTTCGACGCGGACTCTGGAACGCTACGCCAGGGCGACCGGCACGAGGCTCCGGATCACATTGGAAGCC
GCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A126NQV8

Antitoxin

Source ID Structure
AlphaFold DB A0A126P2M2

References