Detailed information of TA system TA060617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1669072..1669667
• Replicon: chromosome
• Accession: NZ_CP014275
• Organism: Martelella sp. AD-3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A127CHF0
• Locus tag: AZF01_RS07715
• Protein ID: WP_024709039.1
• Coordinates: 1669386..1669667 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AZF01_RS07710
• Protein ID: WP_024709040.1
• Coordinates: 1669072..1669368 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZF01_RS24265	1664126..1664332	+	207	WP_161633046.1	phage tail assembly chaperone	-
AZF01_RS07675	1664329..1666251	+	1923	WP_024709047.1	hypothetical protein	-
AZF01_RS07680	1666251..1666598	+	348	WP_024709046.1	phage tail protein	-
AZF01_RS07685	1666595..1666963	+	369	WP_024709045.1	hypothetical protein	-
AZF01_RS07690	1666960..1667715	+	756	WP_024709044.1	phage minor tail protein L	-
AZF01_RS07695	1667729..1668424	+	696	WP_024709043.1	C40 family peptidase	-
AZF01_RS07700	1668412..1668672	-	261	WP_024709042.1	type II toxin-antitoxin system HicB family antitoxin	-
AZF01_RS07705	1668669..1669022	-	354	WP_081725826.1	DUF2442 domain-containing protein	-
AZF01_RS07710	1669072..1669368	-	297	WP_024709040.1	HigA family addiction module antidote protein	Antitoxin
AZF01_RS07715	1669386..1669667	-	282	WP_024709039.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AZF01_RS07720	1669760..1670356	+	597	WP_024709038.1	tail assembly protein	-
AZF01_RS07725	1670375..1673572	+	3198	WP_061449659.1	host specificity protein J	-
AZF01_RS07730	1673569..1674423	+	855	WP_024708615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1644194..1684718	40524

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10510.02 Da
• Isoelectric Point: 9.7897

>T60617 WP_024709039.1 NZ_CP014275:c1669667-1669386 [Martelella sp. AD-3]
MIRTFKDARTEKVASGKAPKGFPANLVKSAIRKLTMIDSAHVLDDLRSPPGNRLEALKGDRQGKHSIRINDQWRICFVWH
EGGAEDVEIVDYH

Nucleotide

• Length: 282 bp

>T60617 NZ_CP014275:c1669667-1669386 [Martelella sp. AD-3]
ATGATCCGGACGTTCAAAGACGCCAGAACCGAGAAGGTCGCTTCAGGAAAAGCGCCGAAGGGGTTTCCGGCCAATCTGGT
AAAGTCCGCCATTCGCAAGCTCACCATGATCGATAGCGCGCATGTGCTGGACGATCTGAGATCGCCGCCCGGCAACCGGC
TTGAAGCCCTCAAGGGCGACAGGCAGGGGAAGCATTCCATCCGGATCAACGACCAATGGCGGATCTGTTTTGTCTGGCAT
GAAGGCGGCGCCGAAGATGTCGAGATCGTCGACTATCACTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11046.87 Da
• Isoelectric Point: 7.2034

>AT60617 WP_024709040.1 NZ_CP014275:c1669368-1669072 [Martelella sp. AD-3]
MNTILPPVHPGEILREEYLVPLGMSAGALAKRLHVPRTRIERIVTEQTAITTDTALRLAKFFRTTPEFWLNMQASYDLKT
QAAAMKAELDRIPEIEAA

Nucleotide

• Length: 297 bp

>AT60617 NZ_CP014275:c1669368-1669072 [Martelella sp. AD-3]
ATGAACACCATTCTGCCCCCGGTTCACCCTGGCGAGATCCTGCGCGAGGAATATCTGGTCCCGCTCGGCATGAGCGCCGG
CGCGCTGGCAAAGCGGCTTCATGTGCCGCGCACGCGCATCGAGCGGATCGTCACGGAACAGACGGCCATTACCACCGACA
CGGCTCTTCGTCTGGCGAAGTTCTTCCGCACGACCCCGGAATTCTGGCTGAACATGCAGGCAAGCTATGATCTGAAGACA
CAAGCCGCAGCCATGAAGGCCGAGCTCGACAGAATTCCGGAAATCGAAGCGGCCTAG

Structures

Toxin

• AlphaFold DB: A0A127CHF0