Detailed information of TA system TA060610

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 115316..115737
• Replicon: plasmid F128-(C3026)
• Accession: NZ_CP014273
• Organism: Escherichia coli K-12 strain K-12 C3026

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: C3026_RS24430
• Protein ID: WP_001302184.1
• Coordinates: 115579..115737 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	115316..115514 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3026_RS24400	111246..111785	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
C3026_RS24405	111842..112075	+	234	WP_000005990.1	DUF905 family protein	-
C3026_RS24410	112141..114099	+	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
C3026_RS24415	114154..114588	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
C3026_RS24420	114585..115304	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	115316..115514	+	199	NuclAT_0	-	Antitoxin
-	115316..115514	+	199	NuclAT_0	-	Antitoxin
-	115316..115514	+	199	NuclAT_0	-	Antitoxin
-	115316..115514	+	199	NuclAT_0	-	Antitoxin
C3026_RS24430	115579..115737	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
C3026_RS28630	115975..116334	-	360	Protein_119	hypothetical protein	-
C3026_RS28635	116404..116610	+	207	WP_000547965.1	hypothetical protein	-
C3026_RS24445	116635..116922	+	288	WP_000107542.1	hypothetical protein	-
C3026_RS24450	117041..117862	+	822	WP_001234417.1	DUF945 domain-containing protein	-
C3026_RS24455	118158..118748	-	591	WP_170919186.1	transglycosylase SLT domain-containing protein	-
C3026_RS24460	119081..119464	+	384	WP_001151527.1	relaxosome protein TraM	-
C3026_RS24465	119651..120340	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	1..213924	213924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T60610 WP_001302184.1 NZ_CP014273:115579-115737 [Escherichia coli K-12]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T60610 NZ_CP014273:115579-115737 [Escherichia coli K-12]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT60610 NZ_CP014273:115316-115514 [Escherichia coli K-12]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin