Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3380264..3381063 Replicon chromosome
Accession NZ_CP014272
Organism Escherichia coli K-12 strain K-12 C3026

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag C3026_RS17035 Protein ID WP_000347273.1
Coordinates 3380599..3381063 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag C3026_RS17030 Protein ID WP_001307405.1
Coordinates 3380264..3380599 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3026_RS17015 3376049..3376819 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
C3026_RS17020 3376835..3378169 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
C3026_RS17025 3378544..3380115 + 1572 WP_001273753.1 galactarate dehydratase -
C3026_RS17030 3380264..3380599 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
C3026_RS17035 3380599..3381063 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
C3026_RS17040 3381118..3381927 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
C3026_RS17045 3382176..3383456 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
C3026_RS17050 3383479..3383952 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
C3026_RS17055 3383963..3384334 + 372 Protein_3291 PTS sugar transporter subunit IIC -
C3026_RS17060 3384330..3384887 + 558 Protein_3292 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T60596 WP_000347273.1 NZ_CP014272:3380599-3381063 [Escherichia coli K-12]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T60596 NZ_CP014272:3380599-3381063 [Escherichia coli K-12]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT60596 WP_001307405.1 NZ_CP014272:3380264-3380599 [Escherichia coli K-12]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT60596 NZ_CP014272:3380264-3380599 [Escherichia coli K-12]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References