Detailed information of TA system TA060563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 221400..221941
• Replicon: chromosome
• Accession: NZ_CP014272
• Organism: Escherichia coli K-12 strain K-12 C3026

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: Q47149
• Locus tag: C3026_RS01060
• Protein ID: WP_000615983.1
• Coordinates: 221400..221678 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: Q47150
• Locus tag: C3026_RS01065
• Protein ID: WP_000729703.1
• Coordinates: 221681..221941 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3026_RS01045	218982..219560	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
C3026_RS01050	219766..220533	+	768	WP_000333380.1	class II glutamine amidotransferase	-
C3026_RS01055	220504..221244	-	741	WP_001225679.1	murein L,D-transpeptidase	-
C3026_RS01060	221400..221678	-	279	WP_000615983.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
C3026_RS01065	221681..221941	-	261	WP_000729703.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
C3026_RS01070	222151..222900	+	750	WP_000056849.1	C40 family peptidase	-
C3026_RS01075	223076..223573	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
C3026_RS01080	223797..225509	-	1713	Protein_205	flagellar biosynthesis protein FlhA	-
C3026_RS01085	225481..226266	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10846.62 Da
• Isoelectric Point: 9.9941

>T60563 WP_000615983.1 NZ_CP014272:c221678-221400 [Escherichia coli K-12]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T60563 NZ_CP014272:c221678-221400 [Escherichia coli K-12]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9405.80 Da
• Isoelectric Point: 5.0758

>AT60563 WP_000729703.1 NZ_CP014272:c221941-221681 [Escherichia coli K-12]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT60563 NZ_CP014272:c221941-221681 [Escherichia coli K-12]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• PDB: 4Q2U

Antitoxin

• AlphaFold DB: A0A5F1F716