Detailed information of TA system TA060558

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 134362..134783
• Replicon: plasmid F128-(DHB4)
• Accession: NZ_CP014271
• Organism: Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: DHB4_RS23315
• Protein ID: WP_001302184.1
• Coordinates: 134625..134783 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	134362..134560 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DHB4_RS23285	130292..130831	+	540	WP_000290801.1	single-stranded DNA-binding protein	-
DHB4_RS23290	130888..131121	+	234	WP_000005990.1	DUF905 family protein	-
DHB4_RS23295	131187..133145	+	1959	WP_000117173.1	ParB/RepB/Spo0J family partition protein	-
DHB4_RS23300	133200..133634	+	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
DHB4_RS23305	133631..134350	+	720	WP_000116348.1	plasmid SOS inhibition protein A	-
-	134362..134560	+	199	NuclAT_0	-	Antitoxin
-	134362..134560	+	199	NuclAT_0	-	Antitoxin
-	134362..134560	+	199	NuclAT_0	-	Antitoxin
-	134362..134560	+	199	NuclAT_0	-	Antitoxin
DHB4_RS23315	134625..134783	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
DHB4_RS27095	135021..135380	-	360	Protein_138	hypothetical protein	-
DHB4_RS27100	135450..135656	+	207	WP_000547965.1	hypothetical protein	-
DHB4_RS23330	135681..135968	+	288	WP_000107542.1	hypothetical protein	-
DHB4_RS23335	136087..136908	+	822	WP_001234417.1	DUF945 domain-containing protein	-
DHB4_RS23340	137204..137794	-	591	WP_170919186.1	transglycosylase SLT domain-containing protein	-
DHB4_RS23345	138127..138510	+	384	WP_001151527.1	relaxosome protein TraM	-
DHB4_RS23350	138697..139386	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	1..232954	232954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T60558 WP_001302184.1 NZ_CP014271:134625-134783 [Escherichia coli K-12]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T60558 NZ_CP014271:134625-134783 [Escherichia coli K-12]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT60558 NZ_CP014271:134362-134560 [Escherichia coli K-12]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin