Detailed information of TA system TA060555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 7876..8570
• Replicon: plasmid F128-(DHB4)
• Accession: NZ_CP014271
• Organism: Escherichia coli K-12 strain K-12 DHB4

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: DHB4_RS22605
• Protein ID: WP_001263489.1
• Coordinates: 8172..8570 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: DHB4_RS22600
• Protein ID: WP_000554758.1
• Coordinates: 7876..8169 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DHB4_RS22580	3508..4005	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
DHB4_RS22585	4229..5941	-	1713	Protein_6	flagellar biosynthesis protein FlhA	-
DHB4_RS22590	5913..6698	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
DHB4_RS22595	6769..7824	+	1056	WP_001226164.1	DNA polymerase IV	-
DHB4_RS22600	7876..8169	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DHB4_RS22605	8172..8570	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DHB4_RS22610	8580..9032	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
DHB4_RS22615	9350..9556	+	207	Protein_12	RtcB family protein	-
DHB4_RS22620	9552..10073	+	522	Protein_13	peptide chain release factor H	-
DHB4_RS22625	10130..11587	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
DHB4_RS22630	11848..12306	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	12902..12982	+	81	NuclAT_1	-	-
-	12902..12982	+	81	NuclAT_1	-	-
-	12902..12982	+	81	NuclAT_1	-	-
-	12902..12982	+	81	NuclAT_1	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	1..232954	232954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T60555 WP_001263489.1 NZ_CP014271:8172-8570 [Escherichia coli K-12]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T60555 NZ_CP014271:8172-8570 [Escherichia coli K-12]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT60555 WP_000554758.1 NZ_CP014271:7876-8169 [Escherichia coli K-12]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT60555 NZ_CP014271:7876-8169 [Escherichia coli K-12]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3